POLR2F Antikörper (C-Term)

Produktnummer ABIN2667947

Dieser Ratte Monoklonal Antikörper detektiert spezifisch POLR2F in WB, IF, ChIP und IP. Es zeigt Reaktivität gegenüber Proben von Human und Maus. Es wurde in 3+ Publikationen zitiert.

Kurzübersicht für POLR2F Antikörper (C-Term) (ABIN2667947)

Target: POLR2F (Polymerase (RNA) II (DNA Directed) Polypeptide F (POLR2F))

Reaktivität: Human, Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser POLR2F Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Immunoprecipitation (IP)

Klon: 4F8

Produktdetails anti-POLR2F Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: RNA pol II antibody (mAb) (Clone 4F8)

Aufreinigung: Protein G Chromatography

Immunogen: This RNA pol II antibody was raised against a synthetic peptide corresponding to human RNA pol II C-terminal heptad repeat domain.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: For RNA Pol II, we also offer an AbFlex RNA Pol II Recombinant Antibody (rAb). For details, see Catalog No. 91151.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 μg/μL

Buffer: Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Lagerung: -20 °C

Informationen zur Lagerung: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Haltbarkeit: 24 months

Referenzen für anti-POLR2F Antikörper (ABIN2667947)

Catania, Pidoux, Allshire: "Sequence features and transcriptional stalling within centromere DNA promote establishment of CENP-A chromatin." in: PLoS genetics, Vol. 11, Issue 3, pp. e1004986, (2015) (PubMed).

Dahiya, Liu, Nonnemacher, Dampier, Wigdahl: "CCAAT enhancer binding protein and nuclear factor of activated T cells regulate HIV-1 LTR via a novel conserved downstream site in cells of the monocyte-macrophage lineage." in: PLoS ONE, Vol. 9, Issue 2, pp. e88116, (2014) (PubMed).

Chapman, Heidemann, Albert, Mailhammer, Flatley, Meisterernst, Kremmer, Eick: "Transcribing RNA polymerase II is phosphorylated at CTD residue serine-7." in: Science (New York, N.Y.), Vol. 318, Issue 5857, pp. 1780-2, (2007) (PubMed).

Details zu POLR2F

Target: POLR2F (Polymerase (RNA) II (DNA Directed) Polypeptide F (POLR2F))

Andere Bezeichnung: RNA Pol II

Hintergrund: RNA pol II (RNA polymerase II) is responsible for synthesizing messenger RNA in eukaryotes. RNA pol II contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, RNA pol II, in combination with several other polymerase subunits, form the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.

Molekulargewicht: 240 kDa

Gen-ID: 5430

NCBI Accession: NP_000928

Pathways: Regulatorische RNA Pathways