DNMT1 Antikörper (AA 637-651)

Produktnummer ABIN2668504

Es sind 4+ Publikationen für dieses Produkt verfügbar. Der Maus Monoklonal anti-DNMT1 Antikörper wird verwendet zum Nachweis von DNMT1 in Proben von Human und Maus. Er wurde validiert für WB, IHC, IP und ChIP.

Kurzübersicht für DNMT1 Antikörper (AA 637-651) (ABIN2668504)

Target: DNMT1 (DNA (Cytosine-5)-Methyltransferase 1 (DNMT1))

Reaktivität: Human, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser DNMT1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP)

Klon: 60B1220-1

Produktdetails anti-DNMT1 Antikörper

Bindungsspezifität: AA 637-651

Verwendungszweck: DNMT1 antibody (mAb) (Clone 60B1220.1)

Aufreinigung: Protein G Chromatography

Immunogen: This DNMT1 antibody was raised against a peptide corresponding to amino acid residues 637-651 of human DNMT1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 2 - 4 μg/mL dilution For DNMT1, we also offer AbFlex DNMT1 Recombinant Antibody (rAb). For details, see Catalog No. 91175.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 μg/μL

Buffer: PBS and 0.05 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Some products may be shipped at room temperature. This will not affect their stability or performance. Store at 4°C for short term. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Haltbarkeit: 24 months

Referenzen für anti-DNMT1 Antikörper (ABIN2668504)

Liao, Karnik, Gu, Ziller, Clement, Tsankov, Akopian, Gifford, Donaghey, Galonska, Pop, Reyon, Tsai, Mallard, Joung, Rinn, Gnirke, Meissner: "Targeted disruption of DNMT1, DNMT3A and DNMT3B in human embryonic stem cells." in: Nature genetics, Vol. 47, Issue 5, pp. 469-78, (2015) (PubMed).

Singh, Archer: "Analysis of the SWI/SNF chromatin-remodeling complex during early heart development and BAF250a repression cardiac gene transcription during P19 cell differentiation." in: Nucleic acids research, Vol. 42, Issue 5, pp. 2958-75, (2014) (PubMed).

Ganguly, Chen, Shin, Devaskar: "Prenatal caloric restriction enhances DNA methylation and MeCP2 recruitment with reduced murine placental glucose transporter isoform 3 expression." in: The Journal of nutritional biochemistry, Vol. 25, Issue 2, pp. 259-66, (2014) (PubMed).

Schnekenburger, Talaska, Puga: "Chromium cross-links histone deacetylase 1-DNA methyltransferase 1 complexes to chromatin, inhibiting histone-remodeling marks critical for transcriptional activation." in: Molecular and cellular biology, Vol. 27, Issue 20, pp. 7089-101, (2007) (PubMed).

Details zu DNMT1

Target: DNMT1 (DNA (Cytosine-5)-Methyltransferase 1 (DNMT1))

Andere Bezeichnung: DNMT1

Hintergrund: DNMT1 (DNA (cytosine-5)-methyltransferase) proteins are involved in DNA methylation in which a methyl group is added to a cytosine residue on DNA, commonly at the C5 position of a CpG dinucleotide. Three families of DNMTs have been identified: DNMT1, DNMT2, and DNMT3 (comprised of DNMT3A and DNMT3B). DNMT1 is the most abundant DNMT in somatic cells and shows a preference for methylating hemi-methylated DNA. It is considered to be a maintenance DNA methyltransferase that is important in the maintenance of specific patterns of methylation throughout cellular divisions. Methylation of mammalian DNA has long been recognized to play a major role in a number of cellular functions such as embryonic development, genetic imprinting, X chromosome inactivation and the control of gene expression. DNA methylation is generally associated with transcriptional repression.

Molekulargewicht: 190 kDa

Gen-ID: 1786

NCBI Accession: NP_001370

Pathways: SARS-CoV-2 Protein Interaktom, Phosphorylierungen bei SARS-CoV-2 Infektion