Histone H3.X/Y Antikörper

Produktnummer ABIN2668484

Zitiert in 1 Publikation. Der Ratte Monoklonal anti-Histone H3.X/Y Antikörper (Klon 8H6-2111) (ABIN2668484) detektiert spezifisch Histone H3.X/Y in ChIP, ICC, IF und WB. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für Histone H3.X/Y Antikörper (ABIN2668484)

Target: Histone H3.X/Y

Reaktivität: Human

Wirt: Ratte

Klonalität: Monoklonal

Applikation: Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)

Klon: 8H6-2111

Produktdetails

Verwendungszweck: Histone H3.X/Y antibody (mAb) (Clone 8H6-2111)

Aufreinigung: Protein G Chromatography

Immunogen: This H3.X/Y antibody was raised against a synthetic peptide corresponding to amino acids 9 - 20 of human H3.X.

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 μg/μL

Buffer: Purified rat IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Lagerung: -20 °C

Informationen zur Lagerung: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Haltbarkeit: 24 months

Referenzen

Wiedemann, Mildner, Bönisch, Israel, Maiser, Matheisl, Straub, Merkl, Leonhardt, Kremmer, Schermelleh, Hake: "Identification and characterization of two novel primate-specific histone H3 variants, H3.X and H3.Y." in: The Journal of cell biology, Vol. 190, Issue 5, pp. 777-91, (2010) (PubMed).

Antigendetails

Target: Histone H3.X/Y

Hintergrund: Histones H3.X and H3.Y are primate-specific variants of histone H3. H3.X and H3.Y are roughly 80 % conserved compared to histone H3.3 The H3.Y protein is highly expressed in several primary cell lines, cancer cell lines and human brain. Expression of histone H3.Y is induced by stress. Exogenously expressed H3.X and H3.Y are efficiently incorporated into chromatin and exhibit dynamics similar to canonical histone H3. Knockdown of H3.Y results in slowed growth and altered expression of cell cycle related genes.

Molekulargewicht: 17 kDa

Gen-ID: 440093