Histone H2B Antikörper (H2BK46ac)

Produktnummer ABIN2668337

Zitiert in 1 Publikation. Der Kaninchen Polyklonal anti-Histone H2B Antikörper (ABIN2668337) detektiert spezifisch Histone H2B in WB und ChIP. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für Histone H2B Antikörper (H2BK46ac) (ABIN2668337)

Target: Histone H2B

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Histone H2B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Chromatin Immunoprecipitation (ChIP)

Produktdetails anti-Histone H2B Antikörper

Bindungsspezifität: H2BK46ac

Verwendungszweck: Histone H2BK46ac antibody (pAb)

Aufreinigung: Unpurified

Immunogen: This Histone H2B acetyl Lys46 antibody was raised against a peptide containing acetyl-lysine 46 of human histone H2B.

Anwendungsinformationen

Applikationshinweise: ChIP: 5 - 10 µL per ChIP WB: 1:4,000 - 1:10,000 dilution

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Rabbit serum containing 30 % glycerol and 0.035 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Lagerung: -20 °C

Informationen zur Lagerung: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Haltbarkeit: 24 months

Referenzen für anti-Histone H2B Antikörper (ABIN2668337)

Kyriss, Jin, Gallegos, Sanford, Wyrick: "Novel functional residues in the core domain of histone H2B regulate yeast gene expression and silencing and affect the response to DNA damage." in: Molecular and cellular biology, Vol. 30, Issue 14, pp. 3503-18, (2010) (PubMed).

Details zu Histone H2B

Target: Histone H2B

Hintergrund: Histone H2B is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points, it is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, they play a major role in regulating gene expression. Lysine N-e-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in chromatin remodeling and in the regulation of gene expression in various cellular functions. Histone H2A and Histone H2B are acetylated in bulk chromatin by p300 and form acetylated Histone H2A/Histone H2B heterodimers. When DNA associates with intact core histone octamers that contain acetylated H2A/H2B dimers, the inhibition of transcriptional initiation significantly decreases, indicating that acetylation of their lysine residues may mediate transcription.

Molekulargewicht: 15 kDa

Gen-ID: 8348

NCBI Accession: NP_003512