Histone H2B Antikörper (H2BK120ac)

Produktnummer ABIN2668336

Es sind 2+ Publikationen für dieses Produkt verfügbar. Der Kaninchen Polyklonal anti-Histone H2B Antikörper wird verwendet zum Nachweis von Histone H2B in Proben von Human. Er wurde validiert für WB, IF, ChIP, DB und ChIP-seq.

Kurzübersicht für Histone H2B Antikörper (H2BK120ac) (ABIN2668336)

Target: Histone H2B

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Histone H2B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), ChIP DNA-Sequencing (ChIP-seq)

Produktdetails anti-Histone H2B Antikörper

Bindungsspezifität: H2BK120ac

Verwendungszweck: Histone H2BK120ac antibody (pAb)

Aufreinigung: Unpurified

Immunogen: This Histone H2B acetyl Lys120 antibody was raised against a peptide including acetyl-lysine 120 of human histone H2B.

Anwendungsinformationen

Applikationshinweise: ChIP: 10 µL per ChIP ChIP-Seq: 5 µL each WB*: 1:1,000 - 1:2,000 dilution IF: 1:500 dilution The modENCODE and NIH Roadmap Epigenomics Mapping Consortiums have implemented rigorous standardization criteria for all assays and reagents to be used. As part of this initiative, antibody specificity testing and the ability of the antibodies to work in ChIP-Seq were assessed in a large-scale study. This Histone H2B acetyl Lys120 antibody was validated for ChIP-Seq in the study (see reference). *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Rabbit serum containing 30 % glycerol and 0.035 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Lagerung: -20 °C

Informationen zur Lagerung: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Haltbarkeit: 24 months

Referenzen für anti-Histone H2B Antikörper (ABIN2668336)

Alvarez-Saavedra, De Repentigny, Lagali, Raghu Ram, Yan, Hashem, Ivanochko, Huh, Yang, Mears, Todd, Corcoran, Bassett, Tokarew, Kokavec, Majumder, Ioshikhes, Wallace, Kothary, Meshorer, Stopka et al.: "Snf2h-mediated chromatin organization and histone H1 dynamics govern cerebellar morphogenesis and neural maturation. ..." in: Nature communications, Vol. 5, pp. 4181, (2014) (PubMed).

Gatta, Mantovani: "Single nucleosome ChIPs identify an extensive switch of acetyl marks on cell cycle promoters." in: Cell cycle (Georgetown, Tex.), Vol. 9, Issue 11, pp. 2149-59, (2011) (PubMed).

Details zu Histone H2B

Target: Histone H2B

Hintergrund: Histone H2B is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points, it is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, they play a major role in regulating gene expression. Lysine N-e-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in chromatin remodeling and in the regulation of gene expression in various cellular functions. Histone H2A and Histone H2B are acetylated in bulk chromatin by p300 and form acetylated Histone H2A/Histone H2B heterodimers. When DNA associates with intact core histone octamers that contain acetylated H2A/H2B dimers, the inhibition of transcriptional initiation significantly decreases, indicating that acetylation of their lysine residues may mediate transcription.

Molekulargewicht: 15 kDa

Gen-ID: 8348

NCBI Accession: NP_778225