Histone H2B Antikörper (H2BK46me2)

Produktnummer ABIN2668335

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch Histone H2B in WB, IF, ICC und DB. Es zeigt Reaktivität gegenüber Proben von Human.Es wurde in 1 Publikation zitiert.

Kurzübersicht für Histone H2B Antikörper (H2BK46me2) (ABIN2668335)

Target: Histone H2B

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Histone H2B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Dot Blot (DB)

Produktdetails anti-Histone H2B Antikörper

Bindungsspezifität: H2BK46me2

Verwendungszweck: Histone H2BK46me2 antibody (pAb)

Aufreinigung: Unpurified

Immunogen: This Histone H2B dimethyl Lys46 antibody was raised against a peptide containing dimethyl-lysine 46 of human histone H2B.

Anwendungsinformationen

Applikationshinweise: WB: 1:500 - 1:2,000 dilution ICC/IF: 1:250 - 1:1,000 dilution

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Rabbit serum containing 30 % glycerol and 0.035 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Lagerung: -20 °C

Informationen zur Lagerung: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Haltbarkeit: 24 months

Referenzen für anti-Histone H2B Antikörper (ABIN2668335)

Kyriss, Jin, Gallegos, Sanford, Wyrick: "Novel functional residues in the core domain of histone H2B regulate yeast gene expression and silencing and affect the response to DNA damage." in: Molecular and cellular biology, Vol. 30, Issue 14, pp. 3503-18, (2010) (PubMed).

Details zu Histone H2B

Target: Histone H2B

Hintergrund: Histone H2B is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points, it is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, they play a major role in regulating gene expression. The methylation of histones can occur on two different residues: arginine or lysine. Changes in methylation of histone H2B may be involved in heat-shock mechanisms.

Molekulargewicht: 15 kDa

Gen-ID: 8348

NCBI Accession: NP_003512