Histone H2B Antikörper (H2BS14ph)

Produktnummer ABIN2668331

Zitiert in 1 Publikation. Der Maus Monoklonal anti-Histone H2B Antikörper (Klon MABI 0251) (ABIN2668331) detektiert spezifisch Histone H2B in WB, IF und ICC. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für Histone H2B Antikörper (H2BS14ph) (ABIN2668331)

Target: Histone H2B

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Histone H2B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Klon: MABI 0251

Produktdetails anti-Histone H2B Antikörper

Bindungsspezifität: H2BS14ph

Verwendungszweck: Histone H2BS14ph antibody (mAb) (Clone MABI 0251)

Aufreinigung: Protein G Chromatography

Immunogen: This Histone H2B phospho Ser14 antibody was raised against a peptide containing phospho Ser14 of human histone H2B.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.46 μg/μL

Buffer: PBS pH 7.5 containing 30 % glycerol, 0.3M NaCl and 0.035 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Lagerung: -20 °C

Informationen zur Lagerung: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Haltbarkeit: 24 months

Referenzen für anti-Histone H2B Antikörper (ABIN2668331)

Kimura, Takizawa, Allemand, Hori, Iborra, Nozaki, Muraki, Hagiwara, Krainer, Fukagawa, Okawa: "A novel histone exchange factor, protein phosphatase 2Cgamma, mediates the exchange and dephosphorylation of H2A-H2B." in: The Journal of cell biology, Vol. 175, Issue 3, pp. 389-400, (2006) (PubMed).

Details zu Histone H2B

Target: Histone H2B

Hintergrund: Histone H2B is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins. H2B is phosphorylated at serine 14 in response to DNA damage (dependant upon PI3 kinase kinase activity) and during apoptosis.by the Mst1 kinase.

Molekulargewicht: 15 kDa

Gen-ID: 8348

NCBI Accession: NP_778225