EIF2C3 Antikörper (AA 1-175)

Produktnummer ABIN2668249

Es sind 2+ Publikationen für dieses Produkt verfügbar. Der Maus Monoklonal anti-EIF2C3 Antikörper wird verwendet zum Nachweis von EIF2C3 in Proben von Human. Er wurde validiert für IF, ICC, IP und ChIP.

Kurzübersicht für EIF2C3 Antikörper (AA 1-175) (ABIN2668249)

Target: EIF2C3 (Eukaryotic Translation Initiation Factor 2C3 (EIF2C3))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser EIF2C3 Antikörper ist unkonjugiert

Applikation: Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP)

Klon: 4B1-F6

Produktdetails anti-EIF2C3 Antikörper

Bindungsspezifität: AA 1-175

Verwendungszweck: Ago3 antibody (mAb) (Clone 4B1-F6)

Aufreinigung: Protein G Chromatography

Immunogen: This Ago3 (Argonaute 3) antibody was raised against a recombinant protein corresponding to amino acids 1-175 of human Ago3.

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 μg/μL

Buffer: Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Lagerung: -20 °C

Informationen zur Lagerung: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Haltbarkeit: 24 months

Referenzen für anti-EIF2C3 Antikörper (ABIN2668249)

Shang, Zhang, Xu, Xi, Zhang, Wang, Wu: "Ribozyme-enhanced single-stranded Ago2-processed interfering RNA triggers efficient gene silencing with fewer off-target effects." in: Nature communications, Vol. 6, pp. 8430, (2015) (PubMed).

Azuma-Mukai, Oguri, Mituyama, Qian, Asai, Siomi, Siomi: "Characterization of endogenous human Argonautes and their miRNA partners in RNA silencing." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 23, pp. 7964-9, (2008) (PubMed).

Details zu EIF2C3

Target: EIF2C3 (Eukaryotic Translation Initiation Factor 2C3 (EIF2C3))

Andere Bezeichnung: Ago3

Hintergrund: Argonaute 3 (Ago3, aka EIF2C3) is a member of the Argonaute family of proteins involved in RNAi mediated gene silencing through siRNA and miRNA effectors. The Argonaute proteins are part of RISC, the RNAi Induced Silencing Complex. Ago3, however, lacks RNase activity and does not appear to cleave target mRNA molecules. Ago3 has both a PAZ and a PIWI domain, indicative of the Argonaute family of proteins.

Molekulargewicht: 110 kDa

Gen-ID: 192669

NCBI Accession: NP_079128

Pathways: Fc-epsilon Rezeptor Signalübertragung, Regulatorische RNA Pathways, EGFR Signaling Pathway, Neurotrophin Signalübertragung