Nuclear Factor kappa B p50 (NFkB p50) Antikörper

Produktnummer ABIN2666083

Der Maus Monoklonal Anti--Antikörper wurde für BCA validiert. Er ist geeignet, in Proben von Human zu detektieren.

Kurzübersicht für Nuclear Factor kappa B p50 (NFkB p50) Antikörper (ABIN2666083)

Target: Nuclear Factor kappa B p50 (NFkB p50)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Biochemical Assay (BCA)

Klon: 4D1

Produktdetails

Aufreinigung: The antibody was purified by affinity chromatography.

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide. Final antibody concentration is 0.5 mg/mL.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Upon receipt, store between 2°C and 8°C.

Antigendetails

Target: Nuclear Factor kappa B p50 (NFkB p50)

Andere Bezeichnung: NF-B p50

Hintergrund: NF-κB/p50 (nuclear factor kappa light chain enhancer in B cells p50, NF-κB1) is a member of the Rel/dorsal family. This ubiquitously expressed nuclear protein is one subunit of the NF-kappa B complex consisting of a 65 kD transactivating subunit and a 50 kD DNA binding subunit. Both subunits are derived from larger precursor proteins. NF-κB acts as a transcriptional activator and was originally identified as an activator of kappa light chain in B cells. NF-κB/p50 is bound (with p65) to IκB inhibitor in cytoplasm in inactive form. Phosphorylation of IκB releases and NF- κB which goes to the nucleus to activate gene expression. NF-κB can be activated with LPS, TNF-α, phorbol ester, or IL-1. The 4D1 monoclonal antibody reacts with human NF-κB p50 and has been shown to be useful for Western blotting.