CCR4 Antikörper (PE-Cy7)

Produktnummer ABIN2659453

Der Armenischer Hamster Monoklonal Anti-CCR4-Antikörper wurde für IF, IP, ICC und ICFC validiert. Er ist geeignet, CCR4 in Proben von Maus zu detektieren.

Kurzübersicht für CCR4 Antikörper (PE-Cy7) (ABIN2659453)

Target: CCR4 (Chemokine (C-C Motif) Receptor 4 (CCR4))

Reaktivität: Maus

Wirt: Armenischer Hamster

Klonalität: Monoklonal

Konjugat: Dieser CCR4 Antikörper ist konjugiert mit PE-Cy7

Applikation: Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC), Intracellular Flow Cytometry (ICFC)

Klon: 2G12

Produktdetails anti-CCR4 Antikörper (PE-Cy7)

Aufreinigung: The antibody was purified by affinity chromatography, and conjugated with PE/Cy7 under optimal conditions. The solution is free of unconjugated PE/Cy7 and unconjugated antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.2 mg/mL

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from prolonged exposure to light. Do not freeze.

Lagerung: 4 °C

Informationen zur Lagerung: The antibody solution should be stored undiluted between 2°C and 8°C.

Details zu CCR4

Target: CCR4 (Chemokine (C-C Motif) Receptor 4 (CCR4))

Andere Bezeichnung: CD194

Hintergrund: Mouse CCR4 cDNA contains 1531 bp, and encodes a protein of 360 amino acids that is 85 % identical to human CCR4. CCR4 binds CCL17 (TARG) and CCL22 (MDC). Naive T cells, bearing receptors for cutaneous antigens, become activated in skin-draining lymph nodes and express cutaneous lymphocyte antigen (CLA), which confers to these cells the capacity to migrate into the skin to exert their normal effector functions (1). CCR4 and CCR10 play an important role in the ligand-mediated recruitment of T cells into the skin in mice and humans, specifically with regards to tethering, firm adhesion, and subsequent extravasation to the site of injury (2,3). CCR4 is expressed in cutaneous regulatory T cells (Tregs). These cells are crucial for the induction and maintenance of self-tolerance and are present in peripheral tissues such as skin and gut under normal, noninflamed conditions (4).In addition, recruitment of Foxp3+ T regulatory cells mediating allograft tolerance depends on the CCR4 chemokine receptor and its ligand CCL22 (5).