CD301b/MGL2 Antikörper (PE-Cy7)

Produktnummer ABIN2659223

Dieses Anti-CD301b/MGL2-Antikörper ist ein Ratte Monoklonal-Antikörper zur Detektion von CD301b/MGL2 in IHC, WB, CyTOF und ELISA (Capture). Geeignet für Maus.

Kurzübersicht für CD301b/MGL2 Antikörper (PE-Cy7) (ABIN2659223)

Target: CD301b/MGL2 (MGL2) (Macrophage Galactose N-Acetyl-Galactosamine Specific Lectin 2 (MGL2))

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser CD301b/MGL2 Antikörper ist konjugiert mit PE-Cy7

Applikation: Immunohistochemistry (IHC), Western Blotting (WB), Cytometry by Time of Flight (CyTOF), ELISA (Capture)

Klon: URA-1

Produktdetails anti-CD301b/MGL2 Antikörper (PE-Cy7)

Aufreinigung: The antibody was purified by affinity chromatography and conjugated with PE/Cy7 under optimal conditions. The solution is free of unconjugated PE/Cy7 and unconjugated antibody.

Isotyp: IgG2a lambda

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.2 mg/mL

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from prolonged exposure to light. Do not freeze.

Lagerung: 4 °C

Informationen zur Lagerung: The antibody solution should be stored undiluted between 2°C and 8°C.

Details zu CD301b/MGL2

Target: CD301b/MGL2 (MGL2) (Macrophage Galactose N-Acetyl-Galactosamine Specific Lectin 2 (MGL2))

Andere Bezeichnung: CD301b

Hintergrund: Mouse CD301, also known as macrophage galactose-type C-type lectin, has two homologue genes, CD301a (MGL1) and CD301b (MGL2), while there is only one MGL in human and rat. Mouse CD301a and CD301b are ~42 kD type II transmembrane glycoproteins containing a cytoplasmic domain, a transmembrane domain, a neck domain, and a carbohydrate recognition domain (CRD) within each molecule. CD301a is mainly expressed on a subset of macrophages and immature dendritic cells (DCs). CD301b is mainly found on conventional DCs. Although CD301a and CD301b share high amino acid sequence homology (92 % for the intact sequence and 80 % for the CRD), they display different carbohydrate specificities. CD301a was found to be highly specific for Lewis X and Lewis A structures, whereas CD301b, more similar to human MGL, recognizes N-actetylgalactosamine (GalNAc) and galactose, including the O-linked Tn-antigen, TF-antigen, and core 2. So far, CD301a has been reported to be involved in recognition and endocytosis of glycoproteins with terminal Gal/GalNAc moieties. This contributes to defense against tumor cell metastasis, tissue remodeling, and clearance of apoptotic cells in embryos. CD301b is involved in the internalization of soluble polyacrylamide polymers (PAA) with α-GalNAc residues (GalNAc-PAA) in bone marrow derived DCs.