CD43 Antikörper (Activation-Associated Glycoform) (Alexa Fluor 488)

Produktnummer ABIN2657429

Produktdetails anti-CD43 Antikörper (Alexa Fluor 488)

Target: CD43 (SPN) (Sialophorin (SPN))

Bindungsspezifität: Activation-Associated Glycoform

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser CD43 Antikörper ist konjugiert mit Alexa Fluor 488

Applikation: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Aufreinigung: The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 488 under optimal conditions.

Klon: 1B11

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from prolonged exposure to light. Do not freeze.

Lagerung: 4 °C

Informationen zur Lagerung: The antibody solution should be stored undiluted between 2°C and 8°C

Details zu CD43

Target: CD43 (SPN) (Sialophorin (SPN))

Andere Bezeichnung: CD43 (SPN Produkte)

Hintergrund: CD43, also known as Ly-48, Leukosialin, Sialophorin, Leukocyte Sialoglycoprotein, and gp115, is a large single chain of type I transmembrane glycoprotein with abundant O-glycosylation and sialylation sites. Due to variable glycosylation and sialylation, two isoforms of CD43 have been identified. The 115 kD glycoform of CD43 is expressed on most hematopietic cells including T lymphocytes, NK cells, monocytes, granulocytes, platelets, and CD5+ B cells. It is not present on resting B cells and erythrocytes. While the 130 kD glycoform is thought to be activation-associated form primarily expressed on myeloid cells, pre-B cells, and activated T cells. It has been reported that CD43 binds to CD54 and Siglec-1. CD43 plays dual roles in cell adhesion and anti-adhesion, as well as costimulation of T cell activation and survival, and induction of apoptosis of T cells and hematopoietic progenitors. The 1B11 antibody reacts with the activation-associated glycoform of CD43. The epitope recognized by 1B11 is also shared with desialylated CD45RB. This antibody is useful for differentiation of effector CD8 T cells and memory T cells.

Pathways: Regulation of Leukocyte Mediated Immunity