DDIT3 Antikörper

Produktnummer ABIN265429

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch DDIT3 in WB, IF, IHC (p) und EIA. Es zeigt Reaktivität gegenüber Proben von Human, Maus und Ratte.

Kurzübersicht für DDIT3 Antikörper (ABIN265429)

Target: DDIT3 (DNA-Damage-Inducible Transcript 3 (DDIT3))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser DDIT3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Produktdetails anti-DDIT3 Antikörper

Spezifität: This antibody detects endogenous levels of GADD153/DDIT3 protein. (region surrounding Lys121)

Kreuzreaktivität (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Aufreinigung: Affinity chromatography using epitope-specific immunogen

Anwendungsinformationen

Applikationshinweise: ELISA: 1/1000approx. 1/10000. Western blot: 1/500approx. 1/1000. Immunohistochemistry: 1/50approx. 1/200. Immunofluorescence: 1/50approx. 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Phosphate Buffered Saline (PBS), pH ~7.2, 0.05 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu DDIT3

Target: DDIT3 (DNA-Damage-Inducible Transcript 3 (DDIT3))

Andere Bezeichnung: GADD153 / CHOP

Hintergrund: GADD 153 has been described as a growth arrest and DNA damageinducible gene that encodes a C/EBP-related nuclear protein. This protein has also been designated C/EBP-homologous protein (CHOP-10). GADD 153 expression is induced by a variety of cellular stresses, inducing nutrient deprivation and metabolic perturbations. GADD153 functions to block cells in G1 to S phase in cell cycle progression and acts by dimerizing with other C/EBP proteins to direct GADD 153 dimers away from 'classical' C/EBP binding sites, recognizing instead unique 'nonclassical' sites. Thus GADD 153 acts as a negative modulator of C/EBP-like proteins in certain terminally differentiated cells, similar to the regulatory function of Id on the activity of Myo D and Myo D-related proteins involved in the development of muscle cells.Synonyms: C/EBP-homologous protein, C/EBP-homologous protein 10, CHOP-10, CHOP10, DDIT-3, DDIT3, DNA damage-inducible transcript 3, Growth arrest and DNA-damage-inducible protein GADD153

Molekulargewicht: approx. 26 kDa

Gen-ID: 1649

NCBI Accession: NP_001181986

UniProt: P35638

Pathways: Regulation of Muscle Cell Differentiation, ER-Nucleus Signaling, Skeletal Muscle Fiber Development, Cell RedoxHomeostasis