Actin Antikörper

Produktnummer ABIN265299

Der Kaninchen Polyklonal anti-Actin Antikörper (ABIN265299) detektiert spezifisch Actin in WB und IHC (p). Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus und Ratte.

Kurzübersicht für Actin Antikörper (ABIN265299)

Target: Actin (ACTA1) (Actin, alpha 1, Skeletal Muscle (ACTA1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Actin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-Actin Antikörper

Spezifität: This antibody detects endogenous levels of Actin-alpha-1 protein (region surrounding Asp3).

Kreuzreaktivität (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Aufreinigung: Affinity Chromatography using epitope-specific immunogen

Anwendungsinformationen

Applikationshinweise: ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu Actin

Target: Actin (ACTA1) (Actin, alpha 1, Skeletal Muscle (ACTA1))

Andere Bezeichnung: alpha Skeletal Muscle Actin / ACTA1

Hintergrund: All eukaryotic cells express actin, which often constitutes as much as 50 % of total cellular protein. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. While lower eukaryotes, such as yeast, have only one actin gene, higher eukaryotes have several isoforms encoded by a family of genes. At least six types of actin are present in mammalian tissues and fall into three classes. α actin expression is limited to various types of muscle, whereas β and γ are the principle constituents of filaments in other tissues. Members of the small GTPase family regulate the organization of the actin cytoskeleton. Rho controls the assembly of actin stress fibers and focal adhesion, Rac regulates actin filament accumulation at the plasma membrane and Cdc42 stimulates formation of filopodia.

Molekulargewicht: approx. 45 kDa

Gen-ID: 58

NCBI Accession: NP_001091

UniProt: P68133

Pathways: Caspase Kaskade in der Apoptose, Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development