SIAH1 Antikörper
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- Target Alle SIAH1 Antikörper anzeigen
- SIAH1 (E3 Ubiquitin-Protein Ligase SIAH1 (SIAH1))
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Reaktivität
- Human
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Dieser SIAH1 Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB)
- Spezifität
- This antibody detects SIAH1 / E3 Ubiquitin Ligase. Antibody tested on human brain lysate.
- Aufreinigung
- Purified
- Immunogen
- Synthetic peptide derived from the human SIAH-1 protein
- Isotyp
- IgG
- Top Product
- Discover our top product SIAH1 Primärantikörper
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- Applikationshinweise
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Western blot (1-5 μg/mL).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Buffer
- Phosphate buffered saline with 0.08 % sodium azide
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
- Avoid repeated freezing and thawing.
- Lagerung
- -20 °C
- Informationen zur Lagerung
- Store the product (in aliquots) at -20 °C. Can be shipped at 2 - 8 °C.
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- Target
- SIAH1 (E3 Ubiquitin-Protein Ligase SIAH1 (SIAH1))
- Andere Bezeichnung
- SIAH1 (SIAH1 Produkte)
- Synonyme
- SIAH antikoerper, Siah1 antikoerper, Siah1a antikoerper, id:ibd2635 antikoerper, zgc:56026 antikoerper, SIAH1A antikoerper, siah antikoerper, E3 ubiquitin-protein ligase Siah1 antikoerper, E3 ubiquitin-protein ligase siah-1 antikoerper, siah E3 ubiquitin protein ligase 1 antikoerper, siah E3 ubiquitin protein ligase 1 L homeolog antikoerper, Tsp_03055 antikoerper, siah-1 antikoerper, Siah1 antikoerper, siah1 antikoerper, SIAH1 antikoerper, siah1.L antikoerper
- Hintergrund
- Seven in absentia homolog 1 (SIAH-1) is a member of the RING-finger-containing E3 ubiquitin ligases. Alpha-synuclein and synphilin-1 are substrates of SIAH-1. Both proteins are involved in the development of Parkinson's disease (PD). Mutations in Parkin, another E3 ubiquitin ligase which ubiquinates synphilin-1 and glycosylated alpha-synuclein, have been defined as a major cause of autosomal recessive PD. The role of SIAH-1 in PD is highlighted by the fact that SIAH-1 is a component of the Lewy bodies and plays a role in apoptosis caused by nitric oxide (NO) induced oxidative stress. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) a classic glycolytic enzyme, and multi-functional protein. GAPDH plays role as a mediator for cell death. GAPDH translocates to the nucleus under a variety of stressors/conditions, most of which are associated with oxidative stress. Sequential steps lead to nuclear translocation of GAPDH during cell death, 1] a catalytic cysteine in GAPDH (C150 in rat GAPDH) is S-nitrosylated by nitric oxide (NO) which is generated from inducible nitric oxide synthase (iNOS) and/or neuronal NOS (nNOS), 2] the modified GAPDH becomes capable of binding with Siah1, an E3 ubiquitin ligase, and stabilizes it,3] the GAPDH-Siah protein complex translocates to the nucleus, dependent on Siah1's nuclear localization signal, and degrades Siah1's substrates in the nucleus, which results in cytotoxicity.Synonyms: E3 ubiquitin-protein ligase SIAH1, HUMSIAH, Seven in absentia homolog 1, Siah-1, Siah-1a
- Gen-ID
- 6477
- NCBI Accession
- NP_001006611
- UniProt
- Q8IUQ4
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