BUB1B Antikörper (C-Term)

Produktnummer ABIN265006

Dieses Schaf Polyklonal-Antikörper erkennt spezifisch BUB1B in WB und IHC (fro). Er zeigt eine Reaktivität gegenüber Maus.

Kurzübersicht für BUB1B Antikörper (C-Term) (ABIN265006)

Target: BUB1B (Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B))

Reaktivität: Maus

Wirt: Schaf

Klonalität: Polyklonal

Konjugat: Dieser BUB1B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-BUB1B Antikörper

Bindungsspezifität: C-Term

Spezifität: This antibody reacts to Bub-1b (C-term).

Aufreinigung: Purified

Immunogen: Recombinant protein corresponding to amino acids 883 - 1052 of the mouse Bub1b protein.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western Blot: 1 - 10 μg/mL, Detects a 130 kDa band. POSITIVE CONTROL: Mitotic NIH/3T3 cells. Immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: Phosphate buffered saline with 0.08 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: The antibody can be shipped at ambient temperature. Store (in aliquots) at -20 °C only.

Details zu BUB1B

Target: BUB1B (Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B))

Andere Bezeichnung: BUB1B

Hintergrund: Bub1, a mitotic checkpoint gene first identified in yeast, ensures proper chromosome segregation by delaying anaphase until chromosomes are properly aligned on the mitotic spindle. Mutations in the mitotic spindle assembly checkpoint genes, like yeast Bub1, cause cells to exit mitosis without proper chromosome attachment/alignment, resulting in aneuploidy. Aneuploidy is a form of genetic instability involved in cancer. Recently, a novel homologue of the murine Bub1 gene has been identified and named Bub1b. The yeast mitotic checkpoint pathway is unresolved, but the mammalian pathway remains even less clear. It is important to investigate the role of the Bub1 genes in the mammalian checkpoint as they have been implicated in carcinogenesis, namely colon carcinomas. The aneuploidy and chromosome instability characterizing some tumors is thought to be caused by mutations in Bub1.Synonyms: BUB1 beta, BUBR1, MAD3/BUB1-related protein kinase, MAD3L, Mitotic checkpoint kinase MAD3L, Mitotic checkpoint serine/threonine-protein kinase BUB1 beta, Protein SSK1

Gen-ID: 701

NCBI Accession: NP_001202

UniProt: O60566