Methylated Lysine (methylated) Antikörper (HRP)

Produktnummer ABIN264882

Der HRP-konjugierte Kaninchen Polyklonal anti-Methylated Lysine Antikörper (ABIN264882) detektiert spezifisch Methylated Lysine in WB und EIA.

Kurzübersicht für Methylated Lysine (methylated) Antikörper (HRP) (ABIN264882)

Target: Methylated Lysine

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: HRP

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails

Bindungsspezifität: methylated

Aufreinigung: Affinity Chromatography

Immunogen: Methylated KLH

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot (4). ELISA.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.25 mg/mL

Buffer: PBS, 0.091 % Sodium Azide, 50 % Glycerol

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing. Centrifuge vial before opening.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at 2 - 8 °C up to one week or (in aliquots) at -20 °C for longer.

Antigendetails

Target: Methylated Lysine

Substanzklasse: Chemical

Hintergrund: Post-translational modifications of proteins play critical roles in the regulation and function of many known biological processes. Proteins can be post-translationally modified in many different ways, and a common posttranscriptional modification of Lysine involves methylation (1). Lysine can be methylated once, twice or three times by lysine methyltransferases. The transfer of methyl groups from S-adenosyl methionine to histones is catalyzed by enzymes known as histone methyltransferases. Histones which are methylated on certain residues can act epigenetically to repress or activate gene expression (1, 2). The transcriptional repressor SUV39H1 can encode novel enzymes which selectively methylate histone H3 at lysine 9. SUV39H1 places a methyl marker on histone H3, which is then recognized by HP1 through its chromo domain. This model may also explain the stable inheritance of the heterochromatic state (3). Some studies have also speculated a stimulatory role for transcription by methylated histone lyside 4 due to its presence at active transcription sites (4-6).