Der Maus Monoklonal anti-DNA Adenine Methylase Antikörper (Klon RB1) (ABIN264462) detektiert spezifisch DNA Adenine Methylase in WB.
Dieser Antikörper reagiert spezifisch mit Proben aus Bakterien.
Western blot (5-10 μg/mL). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Konzentration
1.0 mg/mL
Buffer
PBS containing 0.08 % Sodium Azide as preservative.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
-20 °C
Informationen zur Lagerung
Store the antibody at -20 °C. Avoid repeated freezing and thawing. Shelf life: One year from despatch.
Haltbarkeit
12 months
Target
DNA Adenine Methylase
Hintergrund
DNA adenine methylase (DAM) is an enzyme that methylates adenine residues in the 4-base pair DNA sequence, GATC. This methylation reaction has been shown to be crucial in a variety of cellular processes in bacteria. These include (i) the control of initiation of DNA replication (GATC occurs 11 times in the highly conserved bacterial origin of replication sequence), (ii) methyl-directed mismatch repair, (iii) control of transposition of certain transposable DNA elements, and (iv) control of gene expression. With regard to gene expression, the recent report by Heithoff et al. (Science, 284:967-970, 1999) showed that the deletion of the DAM gene rendered Salmonella typhimurium totally avirulent.Synonyms: DAM, DNA adenine methyltransferase, Deoxyadenosyl-methyltransferase