Myogenin Antikörper (AA 30-224)

Produktnummer ABIN264431

Der Maus Monoklonal Anti-Myogenin-Antikörper wurde für WB, IF, IP, IHC (p), EIA und EMSA validiert. Er ist geeignet, Myogenin in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für Myogenin Antikörper (AA 30-224) (ABIN264431)

Target: Myogenin (MYOG) (Myogenin (Myogenic Factor 4) (MYOG))

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Myogenin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Electrophoretic Mobility-Shift Assay (EMSA)

Klon: F12B

Produktdetails anti-Myogenin Antikörper

Bindungsspezifität: AA 30-224

Spezifität: Recognizes a protein of 34 kDa, identified as myogenin. Its epitope localizes between aa 158-178 of myogenin. This antibody can immunoprecipitate protein-DNA complex and is the antibody of choice for staining of formalin-fixed, paraffin-embedded tissues.

Produktmerkmale: Synonyms: MYOG, MYF4, Myogenic factor 4, BHLHC3, Class C basic helix-loop-helix protein 3

Aufreinigung: Purified

Immunogen: Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized withrecombinant protein containing rat myogenin amino acids 30-224 and mouse myelomaNS1 cells. Genename: MYOG

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: ELISA. Gel Supershift. Immunohistochemistry on Formalin-fixed, Paraffin-embedded tissue sections (1-2 μg/mL). Immunofluorescence. Immunoprecipitation (2 μg/mL). Western Blot (1 μg/mL). Positive Control: Rhabdomyosarcoma and Wlim's tumors.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: PBS containing 0.08 % Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store the antibody at -20 °C. Avoid repeated freezing and thawing.
Shelf life: One year from despatch.

Haltbarkeit: 12 months

Details zu Myogenin

Target: Myogenin (MYOG) (Myogenin (Myogenic Factor 4) (MYOG))

Andere Bezeichnung: Myogenin

Hintergrund: Myogenin is a member of a family of myogenic regulatory genes, which includes MyoD, myf5 and MRF4. These genes encode a set of transcription factors, which are essential for muscle development. Expression of myogenin is restricted to cells of skeletal muscle origin. It is therefore a useful marker for tumors of the muscle lineage, being strongly expressed in alveolar rhabdomyosarcomas.Synonyms: BHLHC3, Class C basic helix-loop-helix protein 3, MYF4, MYOG, Myogenic factor 4

Gen-ID: 29148

UniProt: P20428

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development