KRT14 Antikörper

Produktnummer ABIN264414

Dieses Anti-KRT14-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von KRT14 in WB, IHC (p) und IHC (fro). Geeignet für Human.

Kurzübersicht für KRT14 Antikörper (ABIN264414)

Target: KRT14 (Keratin 14 (KRT14))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser KRT14 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: DE-SPK14

Produktdetails anti-KRT14 Antikörper

Spezifität: This antibody reacts specifically with Human Cytokeratin-14 (50 kDa) by Immunoblotting. Does not react with other Cytokeratins or other proteins.

Kreuzreaktivität (Details): Species reactivity (tested):Human.

Produktmerkmale: Synonyms: Cytokeratin-14, CK14, Keratin 14, K14, KRT14, Keratin Type I Cytoskeletal 14

Aufreinigung: Protein A/G Chromatography

Immunogen: Synthetic peptide conjugated to KLH corresponding to thecarboxy terminal sequence of human cytokeratin 14(KVVSTHEQVLRTKN)Genename: KRT14

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Western Blot: Detects human cytokeratin 14 at 50 kDa. Immunohistochemistry (Frozen Sections): It provides a positive reaction on basal cells ofnon-keratinizing stratified epithelia, basal cells and suprabasal cells of the epidermis andgingiva, myoepithelial cells and squamous cell carcinomas. Not suitable for use in Paraffin embedded tissues.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS, 0.08 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store the antibody at -20 °C. Avoid repeated freezing and thawing.
Shelf life: One year from despatch.

Haltbarkeit: 12 months

Details zu KRT14

Target: KRT14 (Keratin 14 (KRT14))

Andere Bezeichnung: Cytokeratin 14

Hintergrund: Cytokeratins (CK) are intermediate filaments of epithelial cells, both in keratinizing tissue (ie., skin) and non-keratinizing cells (ie., mesothelial cells). Although not a traditional marker for endothelial cells, cytokeratins have also been found in some microvascular endothelial cells. Atleast 20 different cytokeratins (CK) in the molecular range of 40-70 kDa and isoelectric points of 5-8.5 can be identified using two dimensional gel electrophoresis. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells. Monoclonal antibodies to cytokeratin proteins can be useful markers for tumor identification and classification. Keratin 14 is a member of the keratin family, the most diverse group of intermediate filaments. It is usually found as a heterotetramer with two molecules of keratin 5, a type II keratin. Together they form the cytoskeleton of epithelial cells. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex. Keratin 14 has been studied as a prognostic marker in breast cancer.Synonyms: CK14, Cytokeratin-14, K14, KRT14, Keratin 14, Keratin Type I Cytoskeletal 14

Gen-ID: 3861

UniProt: P02533