RIT2 Antikörper

Produktnummer ABIN264413

Der Maus Monoklonal anti-RIT2 Antikörper wird verwendet zum Nachweis von RIT2 in Proben von Human und Maus. Er wurde validiert für WB und IHC (p).

Kurzübersicht für RIT2 Antikörper (ABIN264413)

Target: RIT2 (Ras-Like Without CAAX 2 (RIT2))

Reaktivität: Human, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser RIT2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: 27G2

Produktdetails anti-RIT2 Antikörper

Spezifität: This antibody detects recombinant Rin protein expressed in 293 cells. Does not cross-react with Rit protein.

Produktmerkmale: Synonyms: RIN, ROC2, GTP-binding protein Rit2, Ras-like protein expressed in neurons, Ras-likewithout CAAX protein 2

Aufreinigung: Purified

Immunogen: Hybridoma produced by the fusion of splenocytes from mice immunized with full-lengthrecombinant human Rin protein and mouse myeloma cells. Genename: RIT2

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Western Blot (0.5-1 μg/mL).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: 20 mM Sodium Phosphate, 150 mM Sodium Chloride, 50 % Glycerol at pH 7.5 and 3 mM Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store the antibody at -20 °C. Avoid repeated freezing and thawing.
Shelf life: One year from despatch.

Haltbarkeit: 12 months

Details zu RIT2

Target: RIT2 (Ras-Like Without CAAX 2 (RIT2))

Andere Bezeichnung: RIT2

Hintergrund: Rit and its neuron-specific homologue Rin, define a recently discovered subfamily of Ras-related GTPases. Rit and Rin are membrane-associated in spite of the fact that they lack a CAAX box or similar C-terminal lipidation motif. Rit and Rin display 64 % amino acid sequence identity and share a unique nine amino acid effector domain (DPTIEDAYK) that is 100 % conserved between the murine and human proteins. Although the effector domain sequences of Rit and Rin are very similar to that of Ras, Rit and Rin have been shown to interact with the known Ras-binding proteins RalGDS, Rlf and AF-6, but not the Raf kinases, RIN1 or the p110 subunit of PI3 kinase. For this reason, it has been suggested that Rit and Rin may play important roles in the regulation of signaling pathways distinct from those controlled by Ras.Synonyms: GTP-binding protein Rit2, RIN, ROC2, Ras-like protein expressed in neurons, Ras-like without CAAX protein 2

Gen-ID: 6014

UniProt: Q99578

Pathways: Neurotrophin Signalübertragung