CD28 Antikörper (APC)

Produktnummer ABIN264248

Der Maus Monoklonal Anti-CD28-Antikörper wurde für FACS validiert. Er ist geeignet, CD28 in Proben von Human zu detektieren.

Kurzübersicht für CD28 Antikörper (APC) (ABIN264248)

Target: CD28

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD28 Antikörper ist konjugiert mit APC

Applikation: Flow Cytometry (FACS)

Klon: B-23

Produktdetails anti-CD28 Antikörper (APC)

Spezifität: This antibody recats with CD28 receptor.

Produktmerkmale: Synonyms: TP44, T-cell-specific surface glycoprotein CD28

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Flow Cytometry. Monitoring of activated T cells in peripheral blood. Study of T lymphocyte cytokine function. Study of B cell activation. Study of Cell-adhesion molecules relating T and B lymphocytes.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protokoll: PBMC: Add 10 ul of MAB/10e6 PBMC in 100 ul PBS. Mix gently and incubate for 15 minutes at 2-8°C. Wash twice with PBS and analyze or fix with 0. 5% v/v of paraformaldehyde in PBS andanalyze.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: PBS containing 0.2 % protein carrier and 0.08 % Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C. Do Not Freeze!
Shelf life: One year from despatch.

Haltbarkeit: 12 months

Details zu CD28

Target: CD28

Andere Bezeichnung: CD28

Hintergrund: Anti-human CD28 binds the 44 kDa MW cell surface protein on the surface of most T cells. CD28 acts as the ligand for the B7/BB-1 Molecule on the surface of activated B cells. B7/BB-1 costimulates T cells through CD28, along with CD2 and CD3.CD28 antigen is a disulfide-linked homodimeric glycoprotein. The CD28 antigen is present on approximately 60 % -80 % T lymphocytes (95 % of CD4 and 50 % of CD8 lymphocytes). CD28 regulates the expression of cytokines by T cells, not only IL-2, but also IL-1 alpha and CSF-1, usually synthesized by accessory cells. CD28 functions as a cell adhesion molecule (CAM) for certain T cell subsets.Synonyms: T-cell-specific surface glycoprotein CD28, TP44

Gen-ID: 940

UniProt: P10747

Pathways: T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response