CD69 Antikörper (CD69)

Details for Product anti-CD69 Antibody No. ABIN263925
Antigen
  • 5830438K24Rik
  • AI452015
  • AIM
  • VEA
  • BL-AC/P26
  • CLEC2C
  • EA1
  • GP32/28
  • MLR-3
  • Cd69 molecule
  • CD69 antigen
  • CD69 molecule
  • Cd69
  • CD69
Reaktivität
Human
197
142
44
8
7
7
7
5
4
2
1
Wirt
Maus
117
88
82
15
Klonalität (Klon)
Monoklonal ()
Konjugat
Dieser CD69 Antikörper ist unkonjugiert
46
40
22
15
6
5
5
4
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Applikation
Functional Studies (Func), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
225
86
58
54
48
29
16
15
13
13
6
6
5
3
1
1
Optionen
Immunogen Anti-µ-stimulated human B lymphocytes
Klon FN50
Isotyp IgG1
Spezifität The antibody recognizes CD69, an lymphocyte early activation marker detected as a 53 kDa band under nonreducing and as 29 + 32 kDa double band under reducing SDS-PAGE conditions.
Produktmerkmale Synonyms: GP32/28, Leu-23, MLR-3, EA1, BL-AC/P26, AIM, Early T-cell activation antigen p60, Earlyactivation antigen CD69, Activation inducer molecule, C-type lectin domain family 2member C
Reinigung Affinity Chromatography on Protein A
Antigen
Andere Bezeichnung CD69 (CD69 Antibody Abstract)
Hintergrund CD69 (C-type lectin domain family 2 C, CLEC2C, also known as AIM) is one of the earliest inducible cell surface molecules acquired during leukocyte activation. This glycoprotein serves as a lectin-type receptor in lymphocytes, NK cells and platelets, it is involved in lymphocyte proliferation. CD69 expression is counteracted on T cells in the AIDS stage of HIV infection, and may be also predictive for clinical response to chemoimmunotherapy.Synonyms: AIM, Activation inducer molecule, BL-AC/P26, C-type lectin domain family 2 member C, EA1, Early T-cell activation antigen p60, Early activation antigen CD69, GP32/28, Leu-23, MLR-3
Gen-ID 969
UniProt Q07108
Applikations-hinweise Flow Cytometry: 5 μg/mL. Western blot: 53 kDa, under reducing conditions it shows two bands of 29 and 32 kDa. Immunohistochemistry on Frozen Sections. Functional Assay: When stimulating peripheral blood T cells, FN50 antibody together withTPA synergistically increases cell volume and RNA/DNA synthesis. Addition of FN50 tostimulated peripheral blood B cells has similar, but weaker effect.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen Nur für Forschungszwecke einsetzbar
Konzentration 1.0 mg/mL
Buffer PBS with 15 mM Sodium Azide, pH ~7.4
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung 4 °C/-20 °C
Informationen zur Lagerung Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Haltbarkeit 12 months
Bilder des Herstellers
Image no. 1 for anti-CD69 (CD69) antibody (ABIN263925) anti-CD69 (CD69) antibody
Produkt verwendet in: Zawada, Mrak, Biedermann, Palmer, Gentleman, Aboud, Griffin et al.: "Loss of angiotensin II receptor expression in dopamine neurons in Parkinson's disease correlates with pathological progression and is accompanied by increases in Nox4- and 8-OH guanosine-related ..." in: Acta neuropathologica communications, Vol. 3, pp. 9, 2015 (PubMed).

Johnson, Zhang, Adams, Phillips, Freitas, Froehner, Green-Church, Montanaro: "Proteomic analysis reveals new cardiac-specific dystrophin-associated proteins." in: PLoS ONE, Vol. 7, Issue 8, pp. e43515, 2012 (PubMed).

Hrdinka, Dráber, Stepánek, Ormsby, Otáhal, Angelisová, Brdicka, Paces, Horejsí, Drbal: "PRR7 is a transmembrane adaptor protein expressed in activated T cells involved in regulation of T cell receptor signaling and apoptosis." in: The Journal of biological chemistry, Vol. 286, Issue 22, pp. 19617-29, 2011 (PubMed).

Krämer, Kess, Kremmer, Schulte-Hostede: "Multi-parameter determination of TNF?, PCT and CRP for point-of-care testing." in: The Analyst, Vol. 136, Issue 4, pp. 692-5, 2011 (PubMed).

Drbal, Moertelmaier, Holzhauser, Muhammad, Fuertbauer, Howorka, Hinterberger, Stockinger, Schütz: "Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement." in: International immunology, Vol. 19, Issue 5, pp. 675-84, 2007 (PubMed).

Tomescu, Chehimi, Maino, Montaner: "NK cell lysis of HIV-1-infected autologous CD4 primary T cells: requirement for IFN-mediated NK activation by plasmacytoid dendritic cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 179, Issue 4, pp. 2097-104, 2007 (PubMed).

Allgemeine Veröffentlichungen Pitsios, Dimitrakopoulou, Tsalimalma, Kordossis, Choremi-Papadopoulou: "Expression of CD69 on T-cell subsets in HIV-1 disease." in: Scandinavian journal of clinical and laboratory investigation, Vol. 68, Issue 3, pp. 233-41, 2008 (PubMed).

Nielsen, Afzelius, Ersbøll, Nielsen, Hansen: "Expression of the activation antigen CD69 predicts functionality of in vitro expanded peripheral blood mononuclear cells (PBMC) from healthy donors and HIV-infected patients." in: Clinical and experimental immunology, Vol. 114, Issue 1, pp. 66-72, 1998 (PubMed).

López-Cabrera, Santis, Fernández-Ruiz, Blacher, Esch, Sánchez-Mateos, Sánchez-Madrid: "Molecular cloning, expression, and chromosomal localization of the human earliest lymphocyte activation antigen AIM/CD69, a new member of the C-type animal lectin superfamily of signal-transmitting receptors." in: The Journal of experimental medicine, Vol. 178, Issue 2, pp. 537-47, 1993 (PubMed).