DLG1 Antikörper (Internal Region)

Produktnummer ABIN263164

Dieses Ziege Polyklonal-Antikörper erkennt spezifisch DLG1 in ELISA, IF und FACS. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für DLG1 Antikörper (Internal Region) (ABIN263164)

Target: DLG1 (Discs, Large Homolog 1 (DLG1))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser DLG1 Antikörper ist unkonjugiert

Applikation: ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Güteklasse: Verified

Produktdetails anti-DLG1 Antikörper

Bindungsspezifität: Internal Region

Verwendungszweck: DLG1

Sequenz: NSKTRDKGEI PDD

Kreuzreaktivität: Human, Maus, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: C-NSKTRDKGEIPDD

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western Blot: Preliminary testing showed a band at approx 150 kDa in lysate of cell line Kelly after 0.3 μg/mL antibody staining (calculated MW of 103 kDa according to NP_004078.2). This molecular weight is observed by other sources. Primary incubation 1 h

Peptide ELISA: antibody detection limit dilution 1:16000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm and ER of HeLa and U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentr

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu DLG1

Target: DLG1 (Discs, Large Homolog 1 (DLG1))

Andere Bezeichnung: DLG1

Hintergrund: DLG1 , discs, large homolog 1 (Drosophila) , DKFZp761P0818 , DKFZp781B0426 , DLGH1 , SAP97 , dJ1061C18.1.1 , hdlg, discs large homolog 1 , discs, large homolog 1 , presynaptic protein SAP97 , synapse-associated protein 97

Gen-ID: 1739

NCBI Accession: NP_001091894, NP_004078

Pathways: Regulation of Actin Filament Polymerization, Cell-Cell Junction Organization, Production of Molecular Mediator of Immune Response