DLG1 Antikörper (Internal Region)

Produktnummer ABIN263164

Dieser Ziege Polyklonal Antikörper detektiert spezifisch DLG1 in ELISA, IF und FACS. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für DLG1 Antikörper (Internal Region) (ABIN263164)

Target: DLG1 (Discs, Large Homolog 1 (DLG1))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser DLG1 Antikörper ist unkonjugiert

Applikation: ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Güteklasse: Verified

Produktdetails anti-DLG1 Antikörper

Bindungsspezifität: Internal Region

Verwendungszweck: DLG1

Sequenz: NSKTRDKGEI PDD

Kreuzreaktivität: Human, Maus, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: C-NSKTRDKGEIPDD

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western Blot: Preliminary testing showed a band at approx 150 kDa in lysate of cell line Kelly after 0.3 μg/mL antibody staining (calculated MW of 103 kDa according to NP_004078.2). This molecular weight is observed by other sources. Primary incubation 1 h

Peptide ELISA: antibody detection limit dilution 1:16000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm and ER of HeLa and U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentr

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu DLG1

Target: DLG1 (Discs, Large Homolog 1 (DLG1))

Andere Bezeichnung: DLG1

Hintergrund: DLG1 , discs, large homolog 1 (Drosophila) , DKFZp761P0818 , DKFZp781B0426 , DLGH1 , SAP97 , dJ1061C18.1.1 , hdlg, discs large homolog 1 , discs, large homolog 1 , presynaptic protein SAP97 , synapse-associated protein 97

Gen-ID: 1739

NCBI Accession: NP_001091894, NP_004078

Pathways: Regulation of Actin Filament Polymerization, Cell-Cell Junction Organization, Production of Molecular Mediator of Immune Response