MADCAM1 Antikörper

Produktnummer ABIN2625029

Produktdetails anti-MADCAM1 Antikörper

Target: MADCAM1 (Mucosal Vascular Addressin Cell Adhesion Molecule 1 (MADCAM1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MADCAM1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Recognizes human mucosal addressin cell adhesion molecule 1 (MAdCAM-1), a 60kD cell surface protein that is involved in lymphocyte trafficking. MAdCAM-1 is expressed on high endothelial venules of Peyers patches and mesenteric lymph nodes. MAdCAM-1 expression has also been reported in the gut lamina propria but clone 314G8 does not recognize MAdCAM-1 in these tissues. Clone Reacts with the ligand-binding first Ig domain and reports suggest that a splice variant exists in the gut which is not recognized by clone 314G8. Clone 314G8 is reported to block the interaction of MAdCAM-1 with alpha 4 beta 7.

Aufreinigung: Protein G purified

Endotoxin-Niveau: Low Endotoxin

Immunogen: Recombinant soluble MAdCAM-1-fc fusion protein.

Type of Immunogen: Fusion protein

Klon: 314G8

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Approved: ELISA, Flo (1:10 - 1:100), IHC, IHC-P (10 μg/mL), WB

Usage: Flow Cytometry: Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunohistochemistry: This product does not require protein digestion pre-treatment of paraffin embedded sections prior to staining. This product does not require antigen retrieval prior to staining of paraffin sections. Western Blot: LS-C44686 detects a band of approximately 60kD. Reducing conditions required. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.4.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Aliquot to avoid freeze-thaw cycles. Store undiluted.

Details zu MADCAM1

Target: MADCAM1 (Mucosal Vascular Addressin Cell Adhesion Molecule 1 (MADCAM1))

Andere Bezeichnung: MADCAM1 / MAdCAM-1 (MADCAM1 Produkte)

Synonyme: MACAM1 antikoerper, AV211525 antikoerper, mucosal vascular addressin cell adhesion molecule 1 antikoerper, MADCAM1 antikoerper, Madcam1 antikoerper

Hintergrund: Name/Gene ID: MADCAM1

Synonyms: MADCAM1, HMAdCAM-1, MACAM1, MAdCAM-1

Gen-ID: 8174

UniProt: Q13477