Fibrinogen Antikörper

Produktnummer ABIN2620902

Der Ziege Polyklonal Anti-Fibrinogen-Antikörper wurde für ELISA, IF und DB validiert. Er ist geeignet, Fibrinogen in Proben von Maus zu detektieren.

Kurzübersicht für Fibrinogen Antikörper (ABIN2620902)

Target: Fibrinogen

Reaktivität: Maus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Fibrinogen Antikörper ist unkonjugiert

Applikation: ELISA, Immunofluorescence (IF), Dot Blot (DB)

Produktdetails anti-Fibrinogen Antikörper

Spezifität: Mouse fibrinogen.

Aufreinigung: Purified IgG

Immunogen: Fibrinogen is isolated from fresh plasma after removing prothrombin. Freund's complete adjuvant is used in the first step of the immunization procedure.

Type of Immunogen: Purified protein

Anwendungsinformationen

Applikationshinweise: Approved: DB, ELISA, IF

Usage: The reactivity of the antiserum is restricted to fibrinogen. In immunoelectrophoresis and radial immunodiffusion (Ouchterlony), using various antiserum concentrations against normal mouse plasma a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified fibrinogen. No reaction is obtained with any other plasma protein component or serum. However, the antiserum may also react with fibrin monomers, circulating fibrinopeptides and fibrin degradation products. As unlabelled primary or secondary antibody reagent for the indirect detection of fibrinogen in mouse cells, tissues and body fluids in immunofluorescence and immunoenzyme methods. for the production of immunoconjugates with a selected marker. to prepare insoluble immunoaffinity adsorbents by coupling to an artificial carrier. as catching or detection reagent in non-isotopic methodology and solid phase immunochemistry (e. g. ELISA, Western blotting). When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration. Typical working dilutions in histochemistry are usually between 1:50 and 1:250. in ELISA and comparable non-precipitating antibody-binding assays between 1:500 and 1:5000.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled water

Konzentration: Lot specific

Buffer: Lyophilized from PBS, pH 7.2. No preservative added.

Konservierungsmittel: Without preservative

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C
Short term: +4°C. Avoid repeat freeze-thaw cycles.

Details zu Fibrinogen

Target: Fibrinogen