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Vimentin Antikörper (C-Term)

Name: Vimentin Antikörper (C-Term)
Brand: antibodies-online
SKU: ABIN2613493
Price: 885.26 EUR
Availability: InStock

Dieses Anti-Vimentin-Antikörper ist ein Ziege Polyklonal-Antikörper zur Detektion von Vimentin in WB, IHC, ELISA, IF und FACS. Geeignet für Human, Maus und Ratte.

ABIN2613493 (0. (Vimentin Antikörper (C-Term))

(ABIN2613493) (5 μg/mL) staining of paraffin embedded Human Kidney. (Vimentin Antikörper (C-Term))

IHC

ABIN2613493 (2µg/ml) staining of HeLa (A) and Jurkat (B) lysates (35µg protein in RIPA buffer). (Vimentin Antikörper (C-Term))

ABIN2613493 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0. (Vimentin Antikörper (C-Term))

Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0. (Vimentin Antikörper (C-Term))

FACS

5 Abbildungen

Produktnummer ABIN2613493

Datenblatt (PDF)

Kurzübersicht für Vimentin Antikörper (C-Term) (ABIN2613493)

Target

Alle Vimentin (VIM) Antikörper anzeigen

Vimentin (VIM)

Reaktivität

Human, Maus, Ratte

Wirt

Ziege

Klonalität

Polyklonal

Konjugat

Dieser Vimentin Antikörper ist unkonjugiert

Applikation

Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Güteklasse

Verified

Discover our top product VIM Primärantikörper

Bindungsspezifität
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C-Term

Verwendungszweck

Vimentin

Sequenz

QVINETSQHH DDLE

Kreuzreaktivität

Rind (Kuh), Hund, Human, Maus, Schwein, Ratte

Aufreinigung

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotyp

IgG
anti-Vimentin (VIM) (AA 371-466) antibody
VIM Reaktivität: Human, Maus, Ratte, Schwein, Rind (Kuh), Affe WB, ELISA, FACS, IHC (p), ICC, IHC (fro), IF (p), IF (cc) Wirt: Kaninchen Polyclonal unconjugated

anti-Vimentin (VIM) antibody
KO Validated VIM Reaktivität: Human WB, IHC, ELISA, IF, IHC (p), ICC, IP, IHC (fro) Wirt: Kaninchen Polyclonal unconjugated

anti-Vimentin (VIM) (AA 435-466), (C-Term) antibody
VIM Reaktivität: Human, Maus, Ratte WB, IHC, IF Wirt: Kaninchen Polyclonal unconjugated

anti-Vimentin (VIM) antibody
VIM Reaktivität: Human, Maus, Ratte, Schwein, Huhn WB, IHC (p), ICC, IP Wirt: Maus Monoclonal VI-10 unconjugated

anti-Vimentin (VIM) antibody
VIM Reaktivität: Säugetier WB, FACS, IHC (p), ICC Wirt: Maus Monoclonal VI-01 unconjugated

anti-Vimentin (VIM) antibody
VIM Reaktivität: Human, Maus WB, IF, FACS, IHC (p) Wirt: Kaninchen Polyclonal RB41034 unconjugated

anti-Vimentin (VIM) (AA 1-200) antibody
VIM Reaktivität: Human WB, IHC, IF Wirt: Kaninchen Polyclonal unconjugated

anti-Vimentin (VIM) antibody
VIM Reaktivität: Human WB, IHC, IF Wirt: Maus Monoclonal C12 unconjugated

anti-Vimentin (VIM) (AA 430-457), (C-Term) antibody
VIM Reaktivität: Human WB, IF, FACS, IHC (p) Wirt: Kaninchen Polyclonal RB16691 unconjugated

anti-Vimentin (VIM) antibody
KO Validated VIM Reaktivität: Human WB, IHC, IF, IP Wirt: Kaninchen Monoclonal unconjugated

Applikationshinweise

Immunohistochemistry: Paraffin embedded Human Kidney. Recommended concentration: 5 μg/mL.
Western Blot: Approx 55 kDa band observed in lysates of cell line HeLa and Jurkat and in Mouse Ovary lysates, and approx. 55-60 kDa band in Rat Ovary lysates (calculated MW of 53.7 kDa according to Human NP_003371.2, Mouse NP_035831.2 and Rat NP_112402.1).
Peptide ELISA: antibody detection limit dilution 1:4000.

Kommentare

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml. Immunofluorescence: Strong expression of the protein seen in the cytoplasm/Intermediate filaments of U2OS cells. Recommended conc

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Konzentration

0.5 mg/mL

Buffer

Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel

Sodium azide

Vorsichtsmaßnahmen

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung

Minimize freezing and thawing.

Lagerung

-20 °C

Informationen zur Lagerung

Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
Target

Vimentin (VIM)

Andere Bezeichnung

VIM

Hintergrund

VIM, vimentin, FLJ36605

Gen-ID

7431, 22352, 81818

NCBI Accession

NP_003371

Pathways

Caspase Kaskade in der Apoptose