ICAM1 Antikörper (AA 313-327)

Produktnummer ABIN2613438

Der Ziege Polyklonal Anti-ICAM1-Antikörper wurde für WB, FACS, ELISA und IF validiert. Er ist geeignet, ICAM1 in Proben von Human zu detektieren.

Kurzübersicht für ICAM1 Antikörper (AA 313-327) (ABIN2613438)

Target: ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser ICAM1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), ELISA, Immunofluorescence (IF)

Güteklasse: Verified

Produktdetails anti-ICAM1 Antikörper

Bindungsspezifität: AA 313-327

Verwendungszweck: ICAM1 (aa313-327)

Sequenz: PNVILTKPEV SEGTE

Spezifität: The immunizing peptide represents part of the extracellular domain, and it does not overlap any known glycosite..

Kreuzreaktivität: Human

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western Blot: Approx 100 kDa band observed in Human Lung lysates (calculated MW of 57.8 kDa according to Human NP_000192.2).This molecular weight is routinely observed by other sources. Recommended concentration: 0.1-0.3 μg/mL. Primary incubation 1 hour at

Peptide ELISA: antibody detection limit dilution 1:128000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A431 cells Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of Jurkat cells and Human peripheral blood monocytes

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu ICAM1

Target: ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))

Andere Bezeichnung: ICAM1

Hintergrund: ICAM1, intercellular adhesion molecule 1, BB2, CD54, P3.58, ICAM-1, cell surface glycoprotein P3.58, human rhinovirus receptor, intercellular adhesion molecule 1 (CD54), human rhinovirus receptor, major group rhinovirus receptor

Gen-ID: 3383

NCBI Accession: NP_000192

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Regulation of Leukocyte Mediated Immunity, Thromboxane A2 Receptor Signaling