EEA1 Antikörper (AA 821-835)

Produktnummer ABIN2613419

Dieses Ziege Polyklonal-Antikörper erkennt spezifisch EEA1 in WB, ELISA und IF. Er zeigt eine Reaktivität gegenüber Human und Maus.

Kurzübersicht für EEA1 Antikörper (AA 821-835) (ABIN2613419)

Target: EEA1 (Early Endosome Antigen 1 (EEA1))

Reaktivität: Human, Maus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser EEA1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Güteklasse: Verified

Produktdetails anti-EEA1 Antikörper

Bindungsspezifität: AA 821-835

Verwendungszweck: EEA1 (aa821-835)

Sequenz: QETKIQHEEL NNRIQ

Kreuzreaktivität: Human

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western Blot: Approx 200 kDa band observed in lysates of cell line HEK293 (calculated MW of 162 kDa according to NP_003557.2). This molecular weight is routinely observed by other sources. Recommended concentration: 0.3-1 μg/mL. Primary incubatiion 1 hour a

Peptide ELISA: antibody detection limit dilution 1:128000.

Kommentare:

Immunofluorescence :Strong expression of the protein seen in the vesicles/cytoplasm of NIH3T3 cells. Recommended concentration: 5μg/ml.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu EEA1

Target: EEA1 (Early Endosome Antigen 1 (EEA1))

Andere Bezeichnung: EEA1

Hintergrund: EEA1, early endosome antigen 1, MST105, MSTP105, ZFYVE2, early endosome antigen 1, 162kD, early endosome-associated protein, endosome-associated protein p162, zinc finger FYVE domain-containing protein 2

Gen-ID: 8411

NCBI Accession: NP_003557

Pathways: Toll-Like Receptors Cascades