Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Spezifität
The antibody detects endogenous levels of PKA alpha/beta CAT only when phosphorylated at threonine 197.
Aufreinigung
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Immunogen
PKA alpha/beta CAT (phospho Thr197) antibody was raised against a peptide sequence around phosphorylation site of threonine 197 (R-T-W (p)-T-L) derived from Human PKA alpha/beta CAT.
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
1 mg/mL
Buffer
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
-20 °C
Informationen zur Lagerung
Store antibody at -20°C for up to one year.
Target
PKA alpha/beta Cat
Hintergrund
Phosphorylates a large number of substrates in the cytoplasm and the nucleus. Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis. Phosphorylates CDC25B, ABL1, NFKB1, CLDN3, PSMC5/RPT6, PJA2, RYR2, RORA and VASP. RORA is activated by phosphorylation. Required for glucose-mediated adipogenic differentiation increase and osteogenic differentiation inhibition from osteoblasts.