D1 Protein of PSII, (PsbA) (C-Term) Antikörper

Produktnummer ABIN2559394

Produktdetails

Target: D1 Protein of PSII, (PsbA)

Bindungsspezifität: C-Term

Reaktivität: Arabidopsis thaliana, Gerste, Marchantia polymorpha

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB)

Kreuzreaktivität (Details): Anti-PsbA antibodies will not detect D2 protein, as the peptide used to generatePsbA antibodies has no homology to the D2 sequence.

Produktmerkmale: Expected / apparent Molecular Weight of the Antigene: 38 / 28-30 kDa

Aufreinigung: serum

Immunogen: KLH-conjugated synthetic peptide derived from available plant, algal and cyanobacterial PsbA sequences, including Arabidopsis thaliana UniProt: A4QJR4, TAIR: AtCg00020 , Oryza sativa P0C434, Populus alba Q14FH6, Physcomitrella patens Q6YXN7, Chlamydomonas reinhardtii P07753, Synechocystis sp. P14660 and many others

Anwendungsinformationen

Applikationshinweise: Recommended Dilution: 1 : 10 000 with standard ECL (WB).
A number of degradation products may be observed when using anti-PsbA antibodies, including products having apparent molecular weights of 24kDa and16kDa. D1 degradation is a complex set of events and the products observed can be influenced by both the extraction procedure and the physiology of the cells prior to harvest. Third, cross-linking may occur between D1 and cytochrome b559,shifting the protein higher in the gel. In cyanobacteria (PCC7942), three different bands were competed out by preincubating the antibody with the PsbA free peptide, indicating that all bands are indeed PsbA and its precursors or breakdown products. Competition assays were also performed with spinach and Chlamydomonas, confirming the identity of PsbA bands.

Kommentare:

The antibody is appropriate for detecting both, 24 kDa or the 10 kDa C-terminal fragments, whichever is generated under given treatment conditions. In our analysis we have seen both, ca. 24 kDa and ca. 10 kDa fragments from different samples, depending on treatments and isolation procedures.Rabbit anti-PsbA antibody can detect more than one band of PsbA protein, e.g. precursor and mature protein as compare to the hen anti-PsbA antibodies AS01 016.This antibody will detect the phosphorylated form of D1as an alternate band to the main band on a high resolution gel.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 10 µL of sterile water

Handhabung: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Lagerung: -20 °C

Referenzen

Pandey, Pandey-Rai: "Modulations of physiological responses and possible involvement of defense-related secondary metabolites in acclimation of Artemisia annua L. against short-term UV-B radiation." in: Planta, Vol. 240, Issue 3, pp. 611-27, (2014) (PubMed).

Lagunas, Román, Andreu, Picorel, Alfonso: "A temporal regulatory mechanism controls the different contribution of endoplasmic reticulum and plastidial ω-3 desaturases to trienoic fatty acid content during leaf development in soybean (Glycine max cv Volania)." in: Phytochemistry, Vol. 95, pp. 158-67, (2013) (PubMed).

Nowack, Grossman: "Trafficking of protein into the recently established photosynthetic organelles of Paulinella chromatophora." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, Issue 14, pp. 5340-5, (2012) (PubMed).

Ueda, Kuniyoshi, Yamamoto, Sugimoto, Ishizaki, Kohchi, Nishimura, Shikanai: "Composition and physiological function of the chloroplast NADH dehydrogenase-like complex in Marchantia polymorpha." in: The Plant journal : for cell and molecular biology, Vol. 72, Issue 4, pp. 683-93, (2012) (PubMed).

Schmied, Hedtke, Grimm: "Overexpression of HEMA1 encoding glutamyl-tRNA reductase." in: Journal of plant physiology, (2011) (PubMed).

Balsemão-Pires, Jaillais, Olson, Andrade, Umen, Chory, Sachetto-Martins: "The Arabidopsis translocator protein (AtTSPO) is regulated at multiple levels in response to salt stress and perturbations in tetrapyrrole metabolism." in: BMC plant biology, Vol. 11, pp. 108, (2011) (PubMed).

Krieger-Liszkay, Ka: "Superoxide anion radicals generated by methylviologen in photosystem I damage photosystem II." in: Physiologia plantarum, (2010) (PubMed).

Antigendetails

Target: D1 Protein of PSII, (PsbA)

Hintergrund: AGI Code: ATCG00020
The psbA gene has been cloned from many species of plants, green algae, and cyanobacteria. The psbA gene is located in the chloroplast genome and encodes for the D1 protein, a core component of Photosystem II. PsbA/D1 is rapidly cycled under illumination in all oxygenic photobionts. Tracking PsbA pools using the Global PsbA antibody can show the functional content of Photosystem II in a wide range of samples. Alternative names: 32 kDa thylakoid membrane protein, photosystem II protein D1

Molekulargewicht: expected: 38 kDa, apparent: 28-30 kDa

UniProt: P14660, P0C434, Q6YXN7, P83755, P07753, Q14FH6