GLN1 Antikörper (all Isoforms)

Produktnummer ABIN2559393

Es gibt 1 Publikation für dieses Produkt. Der Kaninchen Polyklonal anti-GLN1 Antikörper wird verwendet zum Nachweis von GLN1 in Proben von Mais (Zea mays), Spinach und Arabidopsis thaliana. Er wurde validiert für WB.

Kurzübersicht für GLN1 Antikörper (all Isoforms) (ABIN2559393)

Target: GLN1 (Glutamine Synthetase (GLN1))

Reaktivität: Mais (Zea mays), Spinach, Arabidopsis thaliana

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GLN1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-GLN1 Antikörper

Bindungsspezifität: all Isoforms

Spezifität: The antibody will recognize both, cytoplasmic and chloroplastic forms of the GS enzyme.

Produktmerkmale: Expected / apparent Molecular Weight of the Antigene: 39-40 kDa (GLN1,cytoplasmic form), 44-45 kDa (GLN2, chloroplastic form)

Aufreinigung: serum

Immunogen: KLH-conjugated synthetic peptide derived from a wide range of available sequences including all isoforms of Arabidopsis thaliana GLN1###-1,1-###2,1-###3 and 1-4, (At5g37600, At5g66200, At5g17820, At5g16570)

Anwendungsinformationen

Applikationshinweise: Recommended Dilution: 1 : 10000 on 0.5-15 ug protein (WB).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 200 µL of sterile water.

Handhabung: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Lagerung: -20 °C

Referenzen für anti-GLN1 Antikörper (ABIN2559393)

Ortigosa, Valderrama-Martín, Ávila, Cánovas, Cañas: "Understanding plant nitrogen nutrition through a laboratory experiment." in: Biochemistry and molecular biology education : a bimonthly publication of the International Union of Biochemistry and Molecular Biology, Vol. 47, Issue 4, pp. 450-458, (2019) (PubMed).

Details zu GLN1

Target: GLN1 (Glutamine Synthetase (GLN1))

Andere Bezeichnung: GS1 glutamine synthetase

Hintergrund: AGI Code: At5g37600
Glutamine synthetase (GLN or GS) is one of the key enzymes involved in nitrogen metabolism of plants. It catalyses the synthesis of glutamine from glutamate and ammonia in an ATP-dependent reaction. There are two general classes of glutamine synthetase in plants: GLN1, a cytosolic form and GLN2, a chloroplastic form. GLN1 is highly abundant in the vascular elements of roots nodules, flowers and fruits, functioning in the assimilation of ammonium and the biosynthesis of glutamine for nitrogen transport. GLN2 is encoded by a single gene and is highly abundant in leaf mesophyll chloroplasts. Here GLN functions in the assimilation of ammonia produced from photorespiration and the reduction of nitrate in the chloroplasts

Molekulargewicht: 39-40 kDa (GLN1 kDa,cytoplasmic form) , 44-45 kDa (GLN2 kDa, chloroplastic form)

UniProt: Q56WN1, Q9FMD9, Q8LCE1, Q9LVI8

Pathways: Positive Regulation of Peptide Hormone Secretion