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GLN1 Antikörper (all Isoforms)

Der Kaninchen Polyklonal Anti-GLN1-Antikörper wurde für WB validiert. Er ist geeignet, GLN1 in Proben von Arabidopsis thaliana, Spinach und Mais (Zea mays) zu detektieren. Es ist 1 Publikation verfügbar.
Produktnummer ABIN2559393

Kurzübersicht für GLN1 Antikörper (all Isoforms) (ABIN2559393)

Target

Alle GLN1 Antikörper anzeigen
GLN1 (Glutamine Synthetase (GLN1))

Reaktivität

  • 41
  • 37
  • 21
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
Arabidopsis thaliana, Spinach, Mais (Zea mays)

Wirt

  • 53
  • 6
  • 3
  • 1
Kaninchen

Klonalität

  • 55
  • 8
Polyklonal

Konjugat

  • 21
  • 12
  • 8
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser GLN1 Antikörper ist unkonjugiert

Applikation

  • 55
  • 33
  • 18
  • 15
  • 14
  • 13
  • 13
  • 12
  • 9
  • 4
  • 3
  • 3
  • 1
  • 1
Western Blotting (WB)
  • Bindungsspezifität

    • 16
    • 5
    • 4
    • 3
    • 1
    • 1
    • 1
    all Isoforms

    Spezifität

    The antibody will recognize both, cytoplasmic and chloroplastic forms of the GS enzyme.

    Produktmerkmale

    Expected / apparent Molecular Weight of the Antigene: 39-40 kDa (GLN1,cytoplasmic form), 44-45 kDa (GLN2, chloroplastic form)

    Aufreinigung

    serum

    Immunogen

    KLH-conjugated synthetic peptide derived from a wide range of available sequences including all isoforms of Arabidopsis thaliana GLN1###-1,1-###2,1-###3 and 1-4, (At5g37600, At5g66200, At5g17820, At5g16570)
  • Applikationshinweise

    Recommended Dilution: 1 : 10000 on 0.5-15 ug protein (WB).

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Lyophilized

    Rekonstitution

    For reconstitution add 200 µL of sterile water.

    Handhabung

    Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
    Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

    Lagerung

    -20 °C
  • Ortigosa, Valderrama-Martín, Ávila, Cánovas, Cañas: "Understanding plant nitrogen nutrition through a laboratory experiment." in: Biochemistry and molecular biology education : a bimonthly publication of the International Union of Biochemistry and Molecular Biology, Vol. 47, Issue 4, pp. 450-458, (2019) (PubMed).

  • Target

    GLN1 (Glutamine Synthetase (GLN1))

    Andere Bezeichnung

    GS1 glutamine synthetase

    Hintergrund

    AGI Code: At5g37600
    Glutamine synthetase (GLN or GS) is one of the key enzymes involved in nitrogen metabolism of plants. It catalyses the synthesis of glutamine from glutamate and ammonia in an ATP-dependent reaction. There are two general classes of glutamine synthetase in plants: GLN1, a cytosolic form and GLN2, a chloroplastic form. GLN1 is highly abundant in the vascular elements of roots nodules, flowers and fruits, functioning in the assimilation of ammonium and the biosynthesis of glutamine for nitrogen transport. GLN2 is encoded by a single gene and is highly abundant in leaf mesophyll chloroplasts. Here GLN functions in the assimilation of ammonia produced from photorespiration and the reduction of nitrate in the chloroplasts

    Molekulargewicht

    39-40 kDa (GLN1 kDa,cytoplasmic form) , 44-45 kDa (GLN2 kDa, chloroplastic form)

    UniProt

    Q56WN1, Q9FMD9, Q8LCE1, Q9LVI8

    Pathways

    Positive Regulation of Peptide Hormone Secretion
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