Fucose Antikörper

Produktnummer ABIN249420

Zitiert in 1 Publikation. Der Kaninchen Polyklonal anti-Fucose Antikörper (ABIN249420) detektiert spezifisch Fucose in ELISA, IL und WB. Dieser Antikörper reagiert spezifisch mit Proben aus Kieselalge (Diatomeen) und Höhere Pflanzen.

Kurzübersicht für Fucose Antikörper (ABIN249420)

Target: Fucose

Reaktivität: Kieselalge (Diatomeen), Höhere Pflanzen

Wirt: Kaninchen

Klonalität: Polyklonal

Applikation: ELISA, Immunolocalization (IL), Western Blotting (WB)

Produktdetails

Verwendungszweck: Anti-Fucose

Kreuzreaktivität (Details): Higher plants, Pheodactylum tricornutum (diatom)
Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Homologie: Higher plants,Phaeodactylum tricornutum

Aufreinigung: Immunogen affinity purified serum

Immunogen: Core fucose residues bound to the N-glycan in alpha 1

Anwendungsinformationen

Applikationshinweise: 0.5 μg/mL (ELISA), 1 : 40 (IL), 1 μg/10 mL (WB)

Kommentare:

controls:PLA2 (phospholipase 2 from bee venom) which contains only α1.3 fucose, Sigma, product number P9279Type II - horseradish peroxidase which contains β1.2 Xylose and α1.3 fucose, Sigma, product number P8250The antibody does not recognize alpha 1,6-fucose.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 50 μL of sterile water

Buffer: Immunogen affinity purified serum in PBS pH 7.4.

Handhabung: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Lagerung: -20 °C

Informationen zur Lagerung: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Referenzen

Baïet, Burel, Saint-Jean, Louvet, Menu-Bouaouiche, Kiefer-Meyer, Mathieu-Rivet, Lefebvre, Castel, Carlier, Cadoret, Lerouge, Bardor: "N-glycans of Phaeodactylum tricornutum diatom and functional characterization of its N-acetylglucosaminyltransferase I enzyme." in: The Journal of biological chemistry, Vol. 286, Issue 8, pp. 6152-64, (2011) (PubMed).

Antigendetails

Target: Fucose

Hintergrund:

Background: This antibody specifically cross-reacts against fucose residues bound to the protein N-glycans in alpha 1,3. This residue is characteristic of the plant protein N-glycans and is absent in protein N-glycans from animals. This residue is added in the Golgi apparatus.

Additional information: Alpha (1,3) fucose is present not only in plants but also in some invertebrates (such as nematodes, bees, etc,) , However, cross-reaction with glycoproteins from these organisms is weaker than the one observed in plants, This sugar residue does not exist in mammals, in their endogenous glycoproteins

Molekulargewicht: 10 - 100 for various glycoproteins