Sar1 Antikörper

Produktnummer ABIN7477852

Dieses Anti-Sar1-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von Sar1 in EM und WB. Geeignet für Arabidopsis thaliana, Pteris vittata (Chinese ladder brake), Wheat und Mais (Zea mays). Dieses Primary Antibody wurde in 3+ Publikationen zitiert.

Kurzübersicht für Sar1 Antikörper (ABIN7477852)

Target: Sar1 (Secretion-Associated and Ras-Related Protein 1 (Sar1))

Reaktivität: Arabidopsis thaliana, Pteris vittata (Chinese ladder brake), Wheat, Mais (Zea mays)

Wirt: Kaninchen

Klonalität: Polyklonal

Applikation: Electron Microscopy (EM), Western Blotting (WB)

Produktdetails anti-Sar1 Antikörper

Kreuzreaktivität (Details): No cross-reactivity with: Nicotiana tabacum

Produktmerkmale: Expected / apparent Molecular Weight of the Antigene: 21 kDa (Arabidopsis thaliana)
VRN-2 and ZCCT2 full-length proteins were expressed as GST fusion proteins in E. coli and purified through GST sepharose columns. The purified VRN-2 and ZCCT2 proteins were used to test the specificity of the VRN-2 antibody by Western blot analysis. A Western blot experiment showed that the VRN-2antibody was able to differentiate VRN-2 from the ZCCT2 protein.

Aufreinigung: serum

Immunogen: GST fusion of full length recombinant Sar1 of Arabidopsis thaliana Q8VYP7, At3g62560

Anwendungsinformationen

Applikationshinweise: Recommended Dilution: 1 : 500 with standard ECL (WB), 1 : 50 (IG).
For immunogold experiments plant tissue has been fixed withGA in PFA/PIPES. LR White resin has been used. Tested species were : Triticum aestivum, Panicum miliaceum,Panicum maximum, Echinochloa crus-galli Eragrostisneomexicana, Digitaria sanguinalis.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 200 µL of sterile water.

Handhabung: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Lagerung: -20 °C

Referenzen für anti-Sar1 Antikörper (ABIN7477852)

Liu, Fan, Li, Song, Li, Zhang, Xue: "SCF(SLF)-mediated cytosolic degradation of S-RNase is required for cross-pollen compatibility in S-RNase-based self-incompatibility in Petunia hybrida." in: Frontiers in genetics, Vol. 5, pp. 228, (2014) (PubMed).

Hopff, Wienkoop, Lüthje: "The plasma membrane proteome of maize roots grown under low and high iron conditions." in: Journal of proteomics, Vol. 91, pp. 605-18, (2013) (PubMed).

Tnani, López, Jouenne, Vicient: "Protein composition analysis of oil bodies from maize embryos during germination." in: Journal of plant physiology, Vol. 168, Issue 5, pp. 510-3, (2011) (PubMed).

Details zu Sar1

Target: Sar1 (Secretion-Associated and Ras-Related Protein 1 (Sar1))

Hintergrund: AGI Code: At3g62560
Sar1 belongs to a small GTPase superfamily and GTP binging activity. This protein is involved in intracellular protein transport. There are two different non-clathrin-coated vesicles that are responsible for transport between ER and the Golgi. Coat protein complexes involving membrane-associated GTP binging proteins - Arf1 and Sar1p for COPI and COPII are needed for formation of COP-coated vesicles. Sar1p is a cytosolic protein. It is temporarily recruited onto the membranes of the ER.

Molekulargewicht: 21 kDa (Arabidopsis thaliana)

UniProt: Q8VYP7