Sar1 Antikörper

Produktnummer ABIN7477852

Zitiert in 3+ Publikationen. Der Kaninchen Polyklonal anti-Sar1 Antikörper (ABIN7477852) detektiert spezifisch Sar1 in EM und WB. Dieser Antikörper reagiert spezifisch mit Proben aus Arabidopsis thaliana, Digitaria sanguinalis, Echinochloa crus-galli, Eragrostis neomexicana, Megathyrsus maximus, Petunia hybrida, Proso millet (Panicum miliaceum), Pteris vittata (Chinese ladder brake), Triticum aestivum und Mais (Zea mays).

Kurzübersicht für Sar1 Antikörper (ABIN7477852)

Target: Sar1 (Secretion-Associated and Ras-Related Protein 1 (Sar1))

Reaktivität: Arabidopsis thaliana, Digitaria sanguinalis, Echinochloa crus-galli, Eragrostis neomexicana, Megathyrsus maximus, Petunia hybrida, Proso millet (Panicum miliaceum), Pteris vittata (Chinese ladder brake), Triticum aestivum, Mais (Zea mays)

Wirt: Kaninchen

Klonalität: Polyklonal

Applikation: Electron Microscopy (EM), Western Blotting (WB)

Produktdetails anti-Sar1 Antikörper

Verwendungszweck: Anti-Sar1 | Secretion-associated and Ras-related protein 1

Kreuzreaktivität (Details): Arabidopsis thaliana, Echinochloa crus-galli Eragrostis neomexicana, Digitaria sanguinalis, Pteris vittata (fern), Panicum miliaceum, Panicum maximum, Petunia hybrida, Triticum aestivum, Zea mays
Not reactive in: Nicotiana tabacum

Homologie: Citrus clementina,Medicago truncatula,Brassica rapa,Hordeum vulgare,Solanum tuberosum,Solanum lycopersicum,Populus trichocarpa,Glycine max,Vitis vinifera,Capsella rubella,Oryza sativa,Sorghum bicolor,Brachypodium distachyon,Brassica campestris,Brassica napus,Theobroma cacao,Triticum aestivum,Ricinus communis

Aufreinigung: Serum

Immunogen: GST fusion of full length recombinant Sar1 of Arabidopsis thaliana UniProt: Q8VYP7

Anwendungsinformationen

Applikationshinweise: 1 : 50 (IG), 1 : 500 (WB)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 100 μL of sterile water

Buffer: Serum

Handhabung: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Lagerung: -20 °C

Informationen zur Lagerung: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Referenzen für anti-Sar1 Antikörper (ABIN7477852)

Liu, Fan, Li, Song, Li, Zhang, Xue: "SCF(SLF)-mediated cytosolic degradation of S-RNase is required for cross-pollen compatibility in S-RNase-based self-incompatibility in Petunia hybrida." in: Frontiers in genetics, Vol. 5, pp. 228, (2014) (PubMed).

Hopff, Wienkoop, Lüthje: "The plasma membrane proteome of maize roots grown under low and high iron conditions." in: Journal of proteomics, Vol. 91, pp. 605-18, (2013) (PubMed).

Tnani, López, Jouenne, Vicient: "Protein composition analysis of oil bodies from maize embryos during germination." in: Journal of plant physiology, Vol. 168, Issue 5, pp. 510-3, (2011) (PubMed).

Details zu Sar1

Target: Sar1 (Secretion-Associated and Ras-Related Protein 1 (Sar1))

Hintergrund:

Background: Sar1 belongs to a small GTPase superfamily and GTP binging activity. This protein is involved in intracellular protein transport. There are two different non-clathrin-coated vesicles that are responsible for transport between ER and the Golgi. Coat protein complexes involving membrane-associated GTP binging proteins - Arf1 and Sar1p for COPI and COPII are needed for formation of COP-coated vesicles. Sar1p is a cytosolic protein. It is temporarily recruited onto the membranes of the ER.

Additional information: For immunogold experiments plant tissue has been fixed with GA in PFA/PIPES. LR White resin has been used. Tested species were: Triticum aestivum, Panicum miliaceum, Panicum maximum, Echinochloa crus-galli Eragrostis neomexicana, Digitaria sanguinalis. Publication in preparation.

Molekulargewicht: 21 kDa (Arabidopsis thaliana)

Gen-ID: 825430

UniProt: Q8VYP7