Not reactive in: no confirmed exceptions from predicted reactivity known in the moment
Homologie
red algae, cyanobacteria, cryptomonads
Produktmerkmale
Expected / apparent Molecular Weight of the Antigene: 17-20 and 30-34 kDa
Aufreinigung
serum
Immunogen
native purified B-phycoerythrin of Porphyridium cruentum with attached chromophores
Applikationshinweise
1:1000 - 5000 with standard ECL (WB)
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
For reconstitution add 200 μL of sterile water.
Handhabung
Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.
Lagerung
-20 °C
Informationen zur Lagerung
store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Gantt, Lipschultz: "Phycobilisomes of Porphyridium cruentum: pigment analysis." in: Biochemistry, Vol. 13, Issue 14, pp. 2960-6, (1974) (PubMed).
Target
Phycoerythrobilin and Phycourobilin (B-Pe)
Hintergrund
B-PE is a phycobiliprotein which is a building block of phycobilisomes, the light-harvesting complex, in red algae and some cyanobacteria. Phycobilisomes are attached to the stromal side of the thylakoid membrane. B-phycoerythrin has both phycoerythrobilin (on alpha and beta subunits) and phycourobilin (on the gamma subunits) chromophores.