PCK1 Antikörper

Produktnummer ABIN249350

Es gibt 1 Publikation für dieses Produkt. Der Kaninchen Polyklonal anti-PCK1 Antikörper wird verwendet zum Nachweis von PCK1 in Proben von Maus, Mais (Zea mays), Saccharum, Pineapple, Oryza sativa, Megathyrsus maximus, Panicum virgatum, Miscanthus giganteus, Nannochloropsis oceanica, Setaria viridis, Spartina alterniflora, Spartina patens und Green bean. Er wurde validiert für WB.

Kurzübersicht für PCK1 Antikörper (ABIN249350)

Target: PCK1 (phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1))

Reaktivität: Maus, Mais (Zea mays), Saccharum, Pineapple, Oryza sativa, Megathyrsus maximus, Panicum virgatum, Miscanthus giganteus, Nannochloropsis oceanica, Setaria viridis, Spartina alterniflora, Spartina patens, Green bean

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PCK1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-PCK1 Antikörper

Verwendungszweck: Anti-PEPCK | PEP carboxykinase

Kreuzreaktivität (Details): Ananas comosus, Miscantus giganteus, Mouse, Nannochloropsis oceanica, Oryza sativa, Panicum maximum, Panicum virgatum, Phaseolus vulgaris, Saccharum spp. hybrid clone C91-301,Setaria viridis, Spartina alterniflora, Spartina patens, Zea mays
Not reactive in: Arabidopsis thaliana, Pinus yunnanensis

Homologie: Brassica napus,Chromera velia,Cucumis sativus,Flaveria sp.,Lycopersicon esculentum,Medicago sativa,Oryza sativa,Urochloa panicoides,Zoysia japonica,Zea mays

Aufreinigung: Serum

Immunogen: KLH-conjugated synthetic peptide well conserved in both PEPCK1 and 2 sequences from different plant species including Zea mays Q9SLZ0

Anwendungsinformationen

Applikationshinweise: 1 : 1000 (WB)

Kommentare:

due to the MW of this protein we suggest to use a gradient gel for protein separation and a longer transfer time. Higher protein load 10-20 µg is adviced when working with this antibody.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 200 μL of sterile water

Buffer: Serum

Handhabung: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Lagerung: -20 °C

Informationen zur Lagerung: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Referenzen für anti-PCK1 Antikörper (ABIN249350)

Aragón, Pascual, González, Escalona, Carvalho, Amancio: "The physiology of ex vitro pineapple (Ananas comosus L. Merr. var MD-2) as CAM or C3 is regulated by the environmental conditions: proteomic and transcriptomic profiles." in: Plant cell reports, (2013) (PubMed).

Details zu PCK1

Target: PCK1 (phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1))

Andere Bezeichnung: PEPCK

Hintergrund: Phosphoenolpyruvate carboxykinase (PEPCK, PEP carboxykinase) is an enzyme that catalyses the conversion of oxaloacetate and ATP to phosphoenelpyruvate, carbon dioxide and ADP. PEPCK is encoded by two genes in plants: pck1 and pck2.

Molekulargewicht: 73 | 78 kDa

Gen-ID: 541622

UniProt: Q9SLZ0

Pathways: Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis