PCK1 Antikörper

Produktnummer ABIN249350

Der Kaninchen Polyklonal Anti-PCK1-Antikörper wurde für WB validiert. Er ist geeignet, PCK1 in Proben von Maus, Mais (Zea mays), Green bean und Miscanthus giganteus zu detektieren. Es ist 1 Publikation verfügbar.

Kurzübersicht für PCK1 Antikörper (ABIN249350)

Target: PCK1 (phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1))

Reaktivität: Maus, Mais (Zea mays), Green bean, Miscanthus giganteus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PCK1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-PCK1 Antikörper

Kreuzreaktivität (Details): No cross-reactivity with: Arabidopsis thaliana

Produktmerkmale: Expected / apparent Molecular Weight of the Antigene: 73 / 78 kDa

Aufreinigung: serum

Immunogen: KLH-conjugated synthetic peptide well conserved in both PEPCK1 and 2 sequences from different plant species including Zea mays Q9SLZ0

Anwendungsinformationen

Applikationshinweise: Recommended Dilution: 1 : 1000 with standard ECL (WB) MW 73 | 78 kDa.

Kommentare:

due to the MW of this protein we suggest to use a gradient gel for protein separation and a longer transfer time. Higher protein load 10-20 µg is adviced when working with this antibody.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 200 µL of sterile water.

Handhabung: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Lagerung: -20 °C

Referenzen für anti-PCK1 Antikörper (ABIN249350)

Aragón, Pascual, González, Escalona, Carvalho, Amancio: "The physiology of ex vitro pineapple (Ananas comosus L. Merr. var MD-2) as CAM or C3 is regulated by the environmental conditions: proteomic and transcriptomic profiles." in: Plant cell reports, (2013) (PubMed).

Details zu PCK1

Target: PCK1 (phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1))

Andere Bezeichnung: PEPCK

Hintergrund: Phosphoenolpyruvate carboxykinase (PEPCK, PEP carboxykinase) Is an enzyme that catalyses the conversion of oxaloacetate and ATP to phosphoenelpyruvate, carbon dioxide and ADP. PEPCK is encoded by two genes in plants: pck1 and pck2. Both genes are nuclear, located on chromosome 4 and 5 respectively (in Arabidopsis). The protein products, PEPCK1 and PEPCK2, are both highly conserved in the model plant, Arabidopsis thaliana.

Molekulargewicht: expected: 73 kDa, apparent: 78 kDa

UniProt: Q9SLZ0

Pathways: Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis