Xylose Antikörper

Produktnummer ABIN249329

Der Kaninchen Polyklonal anti-Xylose Antikörper wird verwendet zum Nachweis von Xylose in Proben von Chlamydomonas und Höhere Pflanzen. Er wurde validiert für WB.

Kurzübersicht für Xylose Antikörper (ABIN249329)

Target: Xylose

Reaktivität: Chlamydomonas, Höhere Pflanzen

Wirt: Kaninchen

Klonalität: Polyklonal

Applikation: Western Blotting (WB)

Produktdetails

Verwendungszweck: Anti-Xylose

Spezifität: Antibody binds to heavy chains of Goat IgG and light chains of all Goat immunoglobuli

Kreuzreaktivität (Details): Higher plants and algae
Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Homologie: Higher plants

Aufreinigung: Immunogen affinity purified serum

Immunogen: xylose residues bound to the N-glycan in beta 1

Anwendungsinformationen

Applikationshinweise: 1 μg/mL (ELISA), 2 μg/10 mL incubation buffer (WB)

Kommentare:

negative control: Fetuin, a glycoprotein containing fucose linked in alpha 1.6 and no xylose, Sigma, product number F3385.positive control: Type II - horseradish peroxidase which contains β1.2 Xylose and α1.3 fucose, Sigma, product number P8250

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 50 μL of sterile water

Buffer: Immunogen affinity purified serum in PBS pH 7.4.

Handhabung: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Lagerung: -20 °C

Informationen zur Lagerung: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Antigendetails

Target: Xylose

Hintergrund:

Background: This antibody specifically cross-reacts against xylose residues bound to the protein N-glycans in beta1,2. This residue is characterisitc of the plant protein N-glycans and is absent in protein N-glycans from animals. This residue is added in the Golgi apparatus.

Additional information: Beta (1,2) xylose is present exclusively in plant N-glycans so antibodies against this sugar moiety should not cross-react with any mammal glycoprotein.This antibody do not bind free D-xylose. This antibody does not seem to work in immunolocalization.

Molekulargewicht: 10-100 for various glycoproteins