SLC9A3 Antikörper (PE)

Produktnummer ABIN2486551

Produktdetails anti-SLC9A3 Antikörper (PE)

Target: SLC9A3 (Solute Carrier Family 9 (Sodium/hydrogen Exchanger), Member 3 (SLC9A3))

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SLC9A3 Antikörper ist konjugiert mit PE

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)

Spezifität: Most abundant in colon and small intestine, followed by kidney and stomach. In kidney, expressed in proximal tubules and outer medulla (at protein level),Detects ~84 kDa.

Kreuzreaktivität: Maus, Ratte

Aufreinigung: Protein A Purified

Immunogen: Synthetic peptide mapping to AA 809 to 831 of rat sequence

Anwendungsinformationen

Applikationshinweise:

• WB (1:1000)
• IHC (1:25)
• optimal dilutions for assays should be determined by the user.

Kommentare:

1 μg/ml of ABIN2486551 was sufficient for detection of HNE3 in 10 μg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Conjugated antibodies should be stored at 4°C

Details zu SLC9A3

Target: SLC9A3 (Solute Carrier Family 9 (Sodium/hydrogen Exchanger), Member 3 (SLC9A3))

Andere Bezeichnung: NHE3 (SLC9A3 Produkte)

Hintergrund: Sodium-hydrogen exchanger 3 (NHE3, Slc9a3) is an epithelial transport protein that carries out 1:1 exchange of Na+ and H+ across the plasma membrane. It is apically located in the proximal tubule of the kidney, the thick ascending limb of the kidney, and in small intestine (1). NHE3 is phosphorylated and regulated by multiple kinases including PKA, SGK1 and CK2. It can be phosphorylated by calyculin A, and dephosphorylated by PP1 catalytic subunit in vitro (2).

Gen-ID: 24784

NCBI Accession: NP_036786

UniProt: P26433

Pathways: Proton Transport