FKBP4 Antikörper (HRP)

Produktnummer ABIN2486531

Dieses Anti-FKBP4-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von FKBP4 in WB, IHC, IP, IF und ICC. Geeignet für Human.

Kurzübersicht für FKBP4 Antikörper (HRP) (ABIN2486531)

Target: FKBP4 (FK506 Binding Protein 4, 59kDa (FKBP4))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser FKBP4 Antikörper ist konjugiert mit HRP

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC)

Klon: Hi52C

Produktdetails anti-FKBP4 Antikörper (HRP)

Spezifität: Detects ~52 kDa. Heavy chain migrates close to FKBP52 on SDS PAGE.

Kreuzreaktivität: Hund, Hamster, Human, Maus, Ratte

Aufreinigung: Protein G Purified

Immunogen: Synthetic peptide corresponding to the residues of human FKBP52

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise:

• WB (1:2000)
• IHC (1:250)
• ICC/IF (1:1000)
• IP (5 μg)
• optimal dilutions for assays should be determined by the user.

Kommentare:

0.5 μg/ml was sufficient for detection of FKBP52 in 20 μg total protein using WB by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Conjugated antibodies should be stored at 4°C

Details zu FKBP4

Target: FKBP4 (FK506 Binding Protein 4, 59kDa (FKBP4))

Andere Bezeichnung: FKBP52

Hintergrund: HSP90 is crucial to cellular signaling by its regulation of the folding, activity, and stability of a wide range of client proteins. These client protein complexes may also contain one or more cochaperones (1). One class of HSP90-binding cochaperone is composed of proteins with a characteristic tetratricopeptide repeat (TPR) domain that forms an HSP90 binding site. Among the TPR cochaperones of HSP90 are Hop/Sti1, protein phosphatase PP5, and members of both the FK506- and cyclosporin A-binding families of immunophilins (2). FK506-binding protein 51 (FKBP51) and FKBP52 are large molecular weight immunophilins that are part of the mature glucocorticoid receptor (GR) heterocomplex (3). The N terminal domain of each protein binds FK506 and has peptidyl-prolyl isomerase (PPIase) activity that converts prolyl peptide bonds within target proteins from cis- to trans- proline. The C-terminal domains contain the TPR repeats involved in protein-protein interactions with the HSP90 (4). Although FKBP52 and FKBP51 share ~75 % sequence similarity, they affect hormone binding by glucocorticoid receptor in opposing manners and have different HSP90-binding characteristics (3). FK506 binding protein 51 kDa (FKBP51 or otherwise referred to as FKBP54) has been identified as a progestininducible gene. This protein is predominantly expressed in murine T cells but in humans, it is abundantly expressed in numerous tissues at levels many times higher than FKBP12. The FKBP51 gene is known to be induced by glucocorticoids (5).

Gen-ID: 2288

NCBI Accession: NP_002005

UniProt: Q02790

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway