FKBP5 Antikörper (HRP)

Produktnummer ABIN2486514

Dieses Maus Monoklonal-Antikörper erkennt spezifisch FKBP5 in WB, IF und ICC. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für FKBP5 Antikörper (HRP) (ABIN2486514)

Target: FKBP5 (FK506 Binding Protein 5 (FKBP5))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser FKBP5 Antikörper ist konjugiert mit HRP

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Klon: Hi51B

Produktdetails anti-FKBP5 Antikörper (HRP)

Spezifität: Detects ~51 kDa.

Kreuzreaktivität: Hund, Hamster, Human, Maus, Kaninchen, Ratte

Aufreinigung: Protein G Purified

Immunogen: Synthetic peptide corresponding to the residues of human FKBP51

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise:

• WB (1:2000)
• ICC/IF (1:1000)
• optimal dilutions for assays should be determined by the user.

Kommentare:

A 1:2000 dilution was sufficient for detection of FKBP51 in ~50 μg total protein using WB analysis.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Conjugated antibodies should be stored at 4°C

Details zu FKBP5

Target: FKBP5 (FK506 Binding Protein 5 (FKBP5))

Andere Bezeichnung: FKBP51

Hintergrund: HSP90 is crucial to cellular signaling by its regulation of the folding, activity, and stability of a wide range of client proteins. These client protein complexes may also contain one or more cochaperones (1). One class of HSP90-binding cochaperone is composed of proteins with a characteristic tetratricopeptide repeat (TPR) domain that forms an HSP90 binding site. Among the TPR cochaperones of HSP90 are Hop/Sti1, protein phosphatase PP5, and members of both the FK506- and cyclosporin A-binding families of immunophilins (2). FK506-binding protein 51 (FKBP51) and FKBP52 are large molecular weight immunophilins that are part of the mature glucocorticoid receptor (GR) heterocomplex (3). The N terminal domain of each protein binds FK506 and has peptidyl-prolyl isomerase (PPIase) activity that converts prolyl peptide bonds within target proteins from cis- to trans- proline. The C-terminal domains contain the TPR repeats involved in protein-protein interactions with the HSP90 (4). Although FKBP52 and FKBP51 share ~75 % sequence similarity, they affect hormone binding by glucocorticoid receptor in opposing manners and have different HSP90-binding characteristics (3). FK506 binding protein 51 kDa (FKBP51 or otherwise referred to as FKBP54) has been identified as a progestininducible gene. This protein is predominantly expressed in murine T cells but in humans, it is abundantly expressed in numerous tissues at levels many times higher than FKBP12. The FKBP51 gene is known to be induced by glucocorticoids (5).

Gen-ID: 2289

NCBI Accession: NP_001139247

UniProt: Q13451