AQP2 Antikörper (C-Term) (APC)

Produktnummer ABIN2486376

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch AQP2 in WB, IHC, IF und ICC. Er zeigt eine Reaktivität gegenüber Ratte.

Kurzübersicht für AQP2 Antikörper (C-Term) (APC) (ABIN2486376)

Target: AQP2 (Aquaporin 2 (Collecting Duct) (AQP2))

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser AQP2 Antikörper ist konjugiert mit APC

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Produktdetails anti-AQP2 Antikörper (APC)

Bindungsspezifität: C-Term

Spezifität: Detects ~28.8 kDa. May detect larger glycosylated bands ~35-50 kDa.

Kreuzreaktivität: Human, Maus, Ratte

Aufreinigung: Protein A Purified

Immunogen: Produced against the C-terminal peptide (Sequence N-CLKGLEPDTDWEEREVRRRQ) of rat aquaporin 2

Anwendungsinformationen

Applikationshinweise:

• WB (1:2000)
• IHC (1:200)
• ICC/IF (1:400)
• optimal dilutions for assays should be determined by the user.

Kommentare:

0.5 μg/ml of ABIN2486376 was sufficient for detection of aquaporin 2 in 10 μg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Conjugated antibodies should be stored at 4°C

Details zu AQP2

Target: AQP2 (Aquaporin 2 (Collecting Duct) (AQP2))

Andere Bezeichnung: Aquaporin 2

Hintergrund: Aquaporins selectively conduct water molecules in and out of the cell, while preventing the passage of ions and other solutes. Known as water channels, they are integral membrane pore proteins (1, 2). Aquaporin 2 is the vasopressin-regulated water channel of the apical membrane of collecting duct cells. It is located in kidney epithelial cells and usually lies dormant in intracellular vesicle membranes. When it is needed vasopressin binds to the cell surface vasopressin receptor, activating a signaling pathway that cause AQP2 containing vesicles to fuse with the plasma membrane so the AQP2 can be used by the cell (3). Defects in AQP2 area cause of an autosomal dominant form of nephrogenic diabetes insipidus (NDI) (4).

Gen-ID: 25386

NCBI Accession: NP_037041

UniProt: P34080

Pathways: Response to Water Deprivation