DNA/RNA Damage Antikörper (PE)

Produktnummer ABIN2486062

Der Maus Monoklonal Anti--Antikörper wurde für DB, ELISA, FACS, Func, ICC, IF, IHC und IP validiert. Er ist geeignet, in Proben von Alle Spezies zu detektieren.

Kurzübersicht für DNA/RNA Damage Antikörper (PE) (ABIN2486062)

Target: DNA/RNA Damage

Reaktivität: Alle Spezies

Wirt: Maus

Klonalität: Monoklonal

Konjugat: PE

Applikation: Dot Blot (DB), ELISA, Flow Cytometry (FACS), Functional Studies (Func), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Klon: 15A3

Produktdetails

Spezifität: Recognizes markers of oxidative damage to DNA (8-hydroxy-2'-deoxyguanosine, 8-hydroxyguanine and 8-hydroxyguanosine).

Aufreinigung: Protein G Purified

Immunogen: 8-hydroxy-guanosine-BSA and -casein conjugates

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise:

• IHC (1:1000)
• optimal dilutions for assays should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.09 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Antigendetails

Target: DNA/RNA Damage

Substanzklasse: Chemical

Hintergrund: DNA or RNA damage is due to environmental factors and normal metabolic processes inside the cell, that then hinder the ability of the cell to carry out its functions. There are four main types of DNA due to endogenous cellular processes and they are oxidation, alkylation, hydrolysis and mismatch of the bases. During the oxidation of bases, highly reactive chemical entities collectively known as RONS, occurs. RONS stands for reactive oxygen and nitrogen species and includes nitric oxide, superoxide, hydroxyl radical, hydrogen peroxide and peroxynitrite. Numerous studies have shown that RONS causes a variety of issues including DNA damage(1). 8-hydroxyguanine, 8-hydroxy-2'-deoxyguanonsine and 8- hydroxyguanosine are all RNA and DNA markers of oxidative damage. 8-hydroxy-2'-guanosine is produced by reactive oxygen and nitrogen species including hydroxyl radical and peroxynitrite. Specifically its high biological relevance is due to its ability to induce G to T transversions, which is one of the most frequent somatic mutations (2). 8-hydroxy-guanine has been the most frequently studied type of DNA base damage, with studies in diabetes, and cancer. Base modifications of this type arise from radical-induced hydroxylation and cleavage reactions of the purine ring (3, 4). And finally, 8-hydroxy-guanosine, like 8-hydroxy-2'-guanosine, induces a mutagenic transversion of G to T in DNA. Its role has specifically been tested in the development of diabetes, hypertension and strokes (5, 6, and 7).