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DNMT1 Antikörper (AA 637-650) (Biotin)

This anti-DNMT1 antibody (ABIN2486005) is a Mouse Monoclonal antibody detecting DNMT1 in WB, IHC, IP, ChIP. Suitable for Human.
Produktnummer ABIN2486005

Kurzübersicht für DNMT1 Antikörper (AA 637-650) (Biotin) (ABIN2486005)

Target

Alle DNMT1 Antikörper anzeigen
DNMT1 (DNA (Cytosine-5)-Methyltransferase 1 (DNMT1))

Reaktivität

  • 178
  • 42
  • 30
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
Human

Wirt

  • 146
  • 36
  • 1
  • 1
Maus

Klonalität

  • 133
  • 51
Monoklonal

Konjugat

  • 86
  • 12
  • 12
  • 10
  • 8
  • 8
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
Dieser DNMT1 Antikörper ist konjugiert mit Biotin

Applikation

  • 119
  • 69
  • 50
  • 43
  • 29
  • 28
  • 23
  • 14
  • 14
  • 14
  • 11
  • 9
  • 4
  • 3
  • 3
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP)

Klon

60B1220-1
  • Bindungsspezifität

    • 22
    • 21
    • 13
    • 12
    • 11
    • 10
    • 9
    • 8
    • 7
    • 5
    • 4
    • 4
    • 3
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    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
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    • 1
    AA 637-650

    Spezifität

    Detects ~180 kDa. It will cross-react with mouse DNMT1.

    Kreuzreaktivität

    Fisch, Human, Maus, Zebrafisch (Danio rerio)

    Aufreinigung

    Protein G Purified

    Immunogen

    Raised against a synthetic peptide corresponding to amino acids 637-650 of human DNMT1

    Isotyp

    IgG1 kappa
  • Applikationshinweise

    • WB (1:1000)
    • IHC (1:1000)
    • optimal dilutions for assays should be determined by the user.

    Kommentare

    2 μg/ml of ABIN2486005 was sufficient for detection of Dnmt1 in 10 μg of mouse ES cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Liquid

    Konzentration

    1 mg/mL

    Buffer

    PBS, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    4 °C

    Informationen zur Lagerung

    Conjugated antibodies should be stored at 4°C
  • Target

    DNMT1 (DNA (Cytosine-5)-Methyltransferase 1 (DNMT1))

    Andere Bezeichnung

    DNMT1

    Hintergrund

    Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Dnmt1 co-purifies with the retinoblastoma (Rb) tumour suppressor gene product, E2F1, and HDAC1. Dnmt1 also cooperates with Rb to repress transcription from promoters containing E2F-binding sites suggesting a link between DNA methylation, histone deacetylase and sequence-specific DNA binding activity, as well as a growth-regulatory pathway that is disrupted in nearly all cancer cells (1-6).

    Gen-ID

    1786

    NCBI Accession

    NP_001370

    UniProt

    P26358

    Pathways

    SARS-CoV-2 Protein Interaktom, Phosphorylierungen bei SARS-CoV-2 Infektion
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