GABBR1 Antikörper (AA 873-977) (APC)

Produktnummer ABIN2484099

Dieses Maus Monoklonal-Antikörper erkennt spezifisch GABBR1 in WB, IF und ICC. Er zeigt eine Reaktivität gegenüber Ratte.

Kurzübersicht für GABBR1 Antikörper (AA 873-977) (APC) (ABIN2484099)

Target: GABBR1 (gamma-aminobutyric Acid (GABA) B Receptor, 1 (GABBR1))

Reaktivität: Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser GABBR1 Antikörper ist konjugiert mit APC

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Klon: S93A-49

Produktdetails anti-GABBR1 Antikörper (APC)

Bindungsspezifität: AA 873-977

Spezifität: Detects ~115 kDa. No cross-reactivity against GABA(B)R2.

Kreuzreaktivität: Human, Maus, Ratte

Aufreinigung: Protein G Purified

Immunogen: Fusion protein amino acids 873-977 (cytoplasmic C-terminus) of rat GABA(B)R1

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise:

• WB (1:1000)
• optimal dilutions for assays should be determined by the user.

Kommentare:

1 μg/ml of ABIN2484099 was sufficient for detection of GABA(B)R1 in 20 μg of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Conjugated antibodies should be stored at 4°C

Details zu GABBR1

Target: GABBR1 (gamma-aminobutyric Acid (GABA) B Receptor, 1 (GABBR1))

Andere Bezeichnung: GABA B Receptor 1

Hintergrund: GABA (γ-aminobutyric acid) is the primary inhibitory neurotransmitter in the central nervous system and interacts with three different receptors: GABA(A), GABA(B) and GABA(C) receptor. The ionotropic GABA(A) and GABA(C) receptors are ligand-gated ion channels that produce fast inhibitory synaptic transmission. In contrast, the metabotropic GABA(B) receptor is coupled to G proteins that modulate slow inhibitory synaptic transmission (1). Functional GABA(B) receptors form heterodimers of GABA(B)R1 and GABA(B)R2 where GABA(B)R1 binds the ligand and GABA(B)R2 is the primary G protein contact site (2). Two isoforms of GABA(B)R1 have been cloned: GABA(B)R1a is a 130 kD protein and GABA(B)R1b is a 95 kD protein (3). G proteins subsequently inhibit adenyl cylase activity and modulate inositol phospholipid hydrolysis. GABA(B) receptors have both pre- and postsynaptic inhibitions: presynaptic GABA(B) receptors inhibit neurotransmitter release through suppression of high threshold calcium channels, while postsynaptic GABA(B) receptors inhibit through coupled activation of inwardly rectifying potassium channels. In addition to synaptic inhibition, GABA(B) receptors may also be involved in hippocampal long-term potentiation, slow wave sleep and muscle relaxation (1).

Gen-ID: 81657

NCBI Accession: NP_112290

UniProt: Q9Z0U4

Pathways: Positive Regulation of Peptide Hormone Secretion, cAMP Metabolic Process