AMIGO1 Antikörper (AA 554-574) (Atto 488)

Produktnummer ABIN2483802

Dieses Maus Monoklonal-Antikörper erkennt spezifisch AMIGO1 in WB, IHC, ICC und IF. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für AMIGO1 Antikörper (AA 554-574) (Atto 488) (ABIN2483802)

Target: AMIGO1 (Adhesion Molecule with Ig-Like Domain 1 (AMIGO1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser AMIGO1 Antikörper ist konjugiert mit Atto 488

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)

Klon: S86-36

Produktdetails anti-AMIGO1 Antikörper (Atto 488)

Bindungsspezifität: AA 554-574

Spezifität: Detects ~60-80 kDa depending on maturity/glycosylation.

Kreuzreaktivität: Human, Maus, Ratte

Aufreinigung: Protein G Purified

Immunogen: Fusion protein amino acids 554-574 (cytoplasmic C-terminus) of human AMIGO-1

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise:

• WB (1:1000)
• optimal dilutions for assays should be determined by the user.

Kommentare:

1 μg/ml of ABIN2483802 was sufficient for detection of AMIGO-1 in 20 μg of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Conjugated antibodies should be stored at 4°C

Details zu AMIGO1

Target: AMIGO1 (Adhesion Molecule with Ig-Like Domain 1 (AMIGO1))

Andere Bezeichnung: AMIGO1

Hintergrund: The amphoterin-induced gene and ORF (AMIGO) family of proteins consists of AMIGO1, AMIGO2 and AMIGO3. All three members are single pass type I membrane proteins that contain several leucine-rich repeats, one IgG domain and a transmembrane domain. The AMIGO proteins are specifically expressed on fiber tracts of neuronal tissues and participate in their formation. They can form complexes with each other, but can also self-bind. AMIGO1, also designated Alivin2, promotes growth and fasciculation of neurites and plays a role in myelination and fasciculation of developing neural axons. In cerebellar neurons, AMIGO2 (Alivin1) is crucial for depolarization-dependent survival. Similar to AMIGO1 and AMIGO2, AMIGO3 (Alivin3) plays a role in hemophilic and/or heterophilic cell-cell interaction and signal transduction.

Gen-ID: 57463

NCBI Accession: NP_065754

UniProt: Q86WK6