NOTCH2 Antikörper (N-Term)

Produktnummer ABIN2470258

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch NOTCH2 in WB, ELISA, IHC und IF. Es zeigt Reaktivität gegenüber Proben von Human, Hund und Schimpanse.

Kurzübersicht für NOTCH2 Antikörper (N-Term) (ABIN2470258)

Target: NOTCH2 (Notch 2 (NOTCH2))

Reaktivität: Human, Hund, Schimpanse

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NOTCH2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Produktdetails anti-NOTCH2 Antikörper

Bindungsspezifität: N-Term

Aufreinigung: Sterile Filtration

Immunogen: NOTCH2 antibody was raised against amino acid residues 2396 - 2409 of NOTCH2. A residue of cysteine was added to the amino terminal end to facilitate coupling (Human).

Anwendungsinformationen

Applikationshinweise: NOTCH2 antibody can be used in Western Blot starting at 0.5 - 4 μg/mL, and immunohistochemistry starting at 5 μg/mL.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.1 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: As with all antibodies avoid freeze/thaw cycles.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store NOTCH2 antibody at 4 °C or -20 °C.

Details zu NOTCH2

Target: NOTCH2 (Notch 2 (NOTCH2))

Andere Bezeichnung: NOTCH2

Hintergrund: Notch is synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase (S1 cleavage) in the trans-golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase (S3 cleavage) to release the intracellular domain (NICD) from the membrane. Notch functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBP-J kappa and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs.

Gen-ID: 4853

UniProt: Q04721

Pathways: Notch Signalweg, Stem Cell Maintenance