MLF1IP Antikörper (Thr78)

Produktnummer ABIN2470179

Dieses Anti-MLF1IP-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von MLF1IP in WB, ELISA, IHC und IP. Geeignet für Human, Rind (Kuh), Schimpanse und Hund.

Kurzübersicht für MLF1IP Antikörper (Thr78) (ABIN2470179)

Target: MLF1IP (MLF1 Interacting Protein (MLF1IP))

Reaktivität: Human, Rind (Kuh), Schimpanse, Hund

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MLF1IP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)

Produktdetails anti-MLF1IP Antikörper

Bindungsspezifität: Thr78

Spezifität: PThr78

Aufreinigung: Immunoaffinity Chromatography

Immunogen: MLF1IP antibody was raised against amino acids surrounding Thr78 of MLF1IP (Human).

Anwendungsinformationen

Applikationshinweise: MLF1immunoprecipitation antibody can be used in ELISA, Western Blot, immunohistochemistry starting at 5 μg/mL, and immunoprecipitation

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: As with all antibodies avoid freeze/thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: Store MLF1IP antibody at -20 °C prior to opening. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 °C or below.

Details zu MLF1IP

Target: MLF1IP (MLF1 Interacting Protein (MLF1IP))

Andere Bezeichnung: MLF1IP

Hintergrund: Myeloid leukemia factor-1 (MLF1) Interacting Protein (also known as PBIP1, MLF1IP1, KLIP1 or KSHV latent nuclear antigen interacting protein 1) is a novel polo-like kinase 1 (Plk1) substrate. Plk1 phosphorylation of MLF1IP induces ubiquitination and degradation of MLF1IP prior to the metaphase/anaphase transition. Several Plk1-dependent phosphorylation sites have been identified on MLF1IP by mass spectrometry. Mutations of these sites stabilize MLF1IP and inhibit mitotic progression. Subsequent in vitro and in vivo MLF1IP phosphorylation and stability assays have revealed that phosphorylation of Thr78 is critical for triggering Plk1-dependent MLF1IP degradation. Expression of a non-degradable Thr78Ala mutant was sufficient to induce a mitotic block. Timely phosphorylation of MLF1IP on Thr78 by Plk1 is critical for eliminating the MLF1IP-imposed mitotic block prior to anaphase onset. MLF1IP is speculated to be a novel tumor suppressor, whose function is required for proper sister-chromatid separation. Loss of MLF1IP function may result in improper segregation of chromosomes and genomic instability, thus promoting tumorigenesis.

Gen-ID: 79682

UniProt: Q71F23