DEFA1/3 (Internal Region) Antikörper
Reaktivität: Human
ELISA, WB
Wirt: Ziege
Polyclonal
unconjugated
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- Target
- DEFA1/3
- Bindungsspezifität
- Internal Region
- Reaktivität
- Human
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Wirt
- Ziege
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Klonalität
- Polyklonal
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Konjugat
- Unkonjugiert
- Applikation
- ELISA, Western Blotting (WB)
- Spezifität
- This antibody is expected to equally recognize DEFA1 precursor and DEFA3 precursor.
- Aufreinigung
- DEFA1/3 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Immunogen
- DEFA1/3 antibody was raised against a 14 amino acid synthetic peptide near the internal region of DEFA1/3.
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- Applikationshinweise
- ELISA: Antibody detection limit dilution 1:128000 Western Blot: Approximately 10 kDa band observed in human spleen lysates (calculated MW of 10.2 kDa according to NP_004075.1). Recommended concentration: 1-3 μg/mL. an additional band of unknown identity was also consistently observed at 20 kDa . This band was successfully blocked by incubation with the immunizing peptide Immunohistochemistry: In paraffin embedded thymus shows staining of selected cells. Recommended concentration, 4-6 μg/mL.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 500 μg/mL
- Buffer
- Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
- Minimize freezing and thawing.
- Lagerung
- -20 °C
- Informationen zur Lagerung
- Aliquot and store at -20 °C.
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