DEFA1/3 (Internal Region) Antikörper

Produktnummer ABIN2464584

Dieses Anti--Antikörper ist ein Ziege Polyklonal-Antikörper zur Detektion von in ELISA und WB. Geeignet für Human.

Kurzübersicht für DEFA1/3 (Internal Region) Antikörper (ABIN2464584)

Target: DEFA1/3

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Unkonjugiert

Applikation: ELISA, Western Blotting (WB)

Produktdetails

Bindungsspezifität: Internal Region

Spezifität: This antibody is expected to equally recognize DEFA1 precursor and DEFA3 precursor.

Aufreinigung: DEFA1/3 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: DEFA1/3 antibody was raised against a 14 amino acid synthetic peptide near the internal region of DEFA1/3.

Anwendungsinformationen

Applikationshinweise: ELISA: Antibody detection limit dilution 1:128000 Western Blot: Approximately 10 kDa band observed in human spleen lysates (calculated MW of 10.2 kDa according to NP_004075.1). Recommended concentration: 1-3 μg/mL. an additional band of unknown identity was also consistently observed at 20 kDa . This band was successfully blocked by incubation with the immunizing peptide Immunohistochemistry: In paraffin embedded thymus shows staining of selected cells. Recommended concentration, 4-6 μg/mL.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 500 μg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20 °C.

Antigendetails

Target: DEFA1/3

Gen-ID: 1667, 1668

NCBI Accession: NP_004075, NP_005208

UniProt: P59665